ScienCell's GeneQuery™ Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:

• M1 microglia phenotype markers: TNF, NOS2, IL1B
• M2 microglia phenotype markers: IL10, ARG1, MRC1
• Pericyte markers: ACTA2, PDGFRB, CSPG4
• Evaluation of neural differentiation
  • Astrocyte markers:   GFAP, S100B, GLUL
  • Immature neuron markers:   NEUROD1, DCX, TUBB3
  • Mature neuron markers:   MAP2, RBFOX3, SYP
• Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
• Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1

Note : all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Brain Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note : all gene names follow their official symbols by HGNC

 * gene selection may be updated based on new research and development