ScienCell's GeneQuery™ Human Ephrin Signaling qPCR Array Kit (GQH-EPH) profiles 88 key genes involved in ephrin signaling and signal transduction. Ephrin signaling is involved in such developmental processes as axonal guidance, angiogenesis, cell migration, embryonic development, and segmentation. Ephrin ligands and receptors are subdivided into two classes based on their structure and binding affinities: ephrin-A and ephrin-B. A more unique aspect of ephrin signaling is that it can occur bidirectionally expressing as both a “forward signal” and a “reverse signal.” Brief examples of how included genes may be grouped according to their function are shown below:
- Signaling receptors: EPHA2, EPHA4, EPHB1, EPHB2, EPHB4
- Ligands: EFNA1, EFNA2, EFNA5, EFNB1, EFNB2
- Cytoskeletal dynamics: ACTB, ACTG1, ACTN1, CFL1, DNM1
- Vesicle Trafficking: AP2B1, AP2S1, APH1A, CAV1, CLTA
- Hemostasis: FN1, FYN, ITGAVM ITGB1, LYN
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Ephrin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACTB |
AP2S1 |
DNM1 |
EFNB3 |
EPHB2 |
GRIA1 |
LIMK1 |
MYL6 |
PIK3CG |
RAP1A |
TF |
β-actin |
|
B |
ACTG1 |
APH1A |
EFNA1 |
EPHA1 |
EPHB3 |
GRIN1 |
LYN |
MYL9 |
PIK3R1 |
RASA1 |
TIAM1 |
GAPDH |
|
C |
ACTN1 |
CAV1 |
EFNA2 |
EPHA2 |
EPHB4 |
GRIN2B |
MAP2K1 |
NCK1 |
PSEN2 |
RGS3 |
TLN1 |
LDHA |
|
D |
ADAM10 |
CDC42 |
EFNA3 |
EPHA3 |
FN1 |
HRAS |
MAPK1 |
NCK2 |
PSENEN |
RHOA |
VAV2 |
NONO |
|
E |
AP2A1 |
CFL1 |
EFNA4 |
EPHA4 |
FYN |
ITGA5 |
MMP2 |
NCSTN |
PTK2 |
ROCK1 |
VAV3 |
PPIH |
|
F |
AP2A2 |
CLTA |
EFNA5 |
EPHA5 |
GNAI1 |
ITGAV |
MMP9 |
PAK1 |
PXN |
SDC2 |
VCL |
GDC |
|
G |
AP2B1 |
CLTB |
EFNB1 |
EPHA7 |
GRB2 |
ITGB1 |
MYH10 |
PAK2 |
RAC1 |
SRC |
VEGFA |
PPC |
|
H |
AP2M1 |
CLTC |
EFNB2 |
EPHB1 |
GRB7 |
KDR |
MYH9 |
PIK3CA |
RAF1 |
SYNJ1 |
WASL |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK089 |
| 产地 | 美国 |
| 缩写 | GQH-EPH |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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