ScienCell's GeneQuery™ Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:
- M1 microglia phenotype markers: TNF, NOS2, IL1B
- M2 microglia phenotype markers: IL10, ARG1, MRC1
- Pericyte markers: ACTA2, PDGFRB, CSPG4
-
Evaluation of neural differentiation
- Astrocyte markers: GFAP, S100B, GLUL
- Immature neuron markers: NEUROD1, DCX, TUBB3
- Mature neuron markers: MAP2, RBFOX3, SYP
- Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
- Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1
Note : all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Brain Development Markers qPCR Array Plate Layout*
(8 controls in Bold and Italic)
Note : all gene names follow their official symbols by HGNC
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
TNF |
NOS2 |
IL1B |
ACTB |
TNF |
NOS2 |
IL1B |
ACTB |
TNF |
NOS2 |
IL1B |
ACTB |
B |
IL10 |
ARG1 |
MRC1 |
GAPDH |
IL10 |
ARG1 |
MRC1 |
GAPDH |
IL10 |
ARG1 |
MRC1 |
GAPDH |
C |
ACTA2 |
PDGFRB |
CSPG4 |
LDHA |
ACTA2 |
PDGFRB |
CSPG4 |
LDHA |
ACTA2 |
PDGFRB |
CSPG4 |
LDHA |
D |
GFAP |
S100B |
GLUL |
NONO |
GFAP |
S100B |
GLUL |
NONO |
GFAP |
S100B |
GLUL |
NONO |
E |
NEUROD1 |
DCX |
TUBB3 |
PPIH |
NEUROD1 |
DCX |
TUBB3 |
PPIH |
NEUROD1 |
DCX |
TUBB3 |
PPIH |
F |
MAP2 |
RBFOX3 |
SYP |
GDC |
MAP2 |
RBFOX3 |
SYP |
GDC |
MAP2 |
RBFOX3 |
SYP |
GDC |
G |
CHAT |
TH |
MAPT |
PPC |
CHAT |
TH |
MAPT |
PPC |
CHAT |
TH |
MAPT |
PPC |
H |
VCAM1 |
OCLN |
ICAM1 |
NTC |
VCAM1 |
OCLN |
ICAM1 |
NTC |
VCAM1 |
OCLN |
ICAM1 |
NTC |
* gene selection may be updated based on new research and development
货号 | GK127 |
产地 | 美国 |
缩写 | GQH-BDM |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |
货号 | 产品名称 | 规格 | 价格 | 指令 | |
GK101 | 人类Synoviocyte细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK102 | 人类滋养层细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK103 | 人前脂肪细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK105 | 人类ECM降解qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK106 | 人类焦点粘附复合物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK107 | 人内皮素信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK111 | 人凝集素调节免疫应答qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK112 | 人类神经分化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK113 | 人类神经干细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK114 | 人类小胶质细胞极化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK115 | 人类骨髓谱系标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK116 | 人B细胞和T细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK117 | 人胸腺细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK118 | 人类先天淋巴细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK119 | 人类神经祖细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK121 | 人PD-1 / PD-L1检查点途径qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK123 | 人类生长因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK124 | 人类转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK125 | 人干细胞转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK126 | 人类细胞表面标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
GK127 | 人脑发育标记物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |