The colorectum is a major organ in both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colonic epithelial cells (HCoEpiC) can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo. They also respond to a broad array of cytokines with altered gene expression and growth characteristics. In addition, normal colonic epithelial cells were found to express the α3, α5, α6, β1 and β4 integrin. HCoEpiC from ScienCell Research Laboratories are isolated from human colonic tissue. HCoEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCoEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HCoEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoEpiC are guaranteed to further culture for 10 population doublings under the conditions provided by ScienCell Research Laboratories Recommended Medium It is recommended to use Colonic Epithelial Cell Medium (CoEpiCM, Cat. #2951) for culturing HCoEpiC in vitro.