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人类焦点粘附复合物qPCR检测试剂盒
Human Focal Adhesion Complexes qPCR Array Kit
品牌:
ScienCell
货号:
GK106
价格:
$286
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ScienCell's GeneQuery™ Human Focal Adhesion Complexes qPCR Array Kit (GQH-FAC) profiles 88 key genes involved in the regulation and formation of focal adhesions.  Focal adhesions are large macromolecular complexes by which the cell interacts with its extracellular matrix (ECM) environment.  They are involved in such diverse processes as interpreting mechanical force, regulating cell adhesion and motility, and contributing to intracellular signaling transduction.  Integrins and focal adhesion kinase (PTK2) are the main regulatory elements of focal adhesions.  Brief examples of how included genes may be categorized are shown below:

  • FAK signaling: CCND1, FYN, PTK2, RHOA, SRC
  • Cytoskeletal elements: ACTB, ACTN1, ACTN3, FLNA, VCL
  • ECM components: COL1A2, COL2A1, COL4A2, FN1, LAMC3
  • ECM binding elements: ITGA1, ITGA5, ITGAM, ITGB1, ITGB3
  • Signaling transduction genes: ARHGAP5, ELK1, HGF, JUN, KDR

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Focal Adhesion Complexes qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTB

BRAF

COL4A2

FYN

ITGA3

ITGAL

ITGB5

MAP2K1

PAK2

PXN

SHC1

β-actin

B

ACTN1

CAV1

CRK

GRB2

ITGA4

ITGAM

ITGB6

MAPK1

PAK4

RAC1

SHC3

GAPDH

C

ACTN3

CAV2

DOCK1

HGF

ITGA5

ITGAV

ITGB7

MAPK10

PDGFRA

RAF1

SOS1

LDHA

D

AKT1

CAV3

EGFR

HRAS

ITGA6

ITGAX

JUN

MAPK8

PDGFRB

RAP1A

SRC

NONO

E

AKT2

CCND1

ELK1

ILK

ITGA7

ITGB1

KDR

MAPK9

PDPK1

RAPGEF1

TLN1

PPIH

F

AKT3

CDC42

FLNA

ITGA1

ITGA8

ITGB2

LAMA1

MET

PIK3R1

RHOA

VAV3

GDC

G

ARHGAP5

COL1A2

FLT1

ITGA2

ITGAD

ITGB3

LAMB1

MYLK2

PIK3R2

ROCK1

VCL

PPC

H

BCAR1

COL2A1

FN1

ITGA2B

ITGAE

ITGB4

LAMC3

PAK1

PTK2

ROCK2

VTN

NTC

 

 * gene selection may be updated based on new research and development

货号GK106
产地美国
缩写GQH-FAC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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