ScienCell's GeneQuery™ human pluripotent stem cell qPCR array kit (GQH-hPSC) is designed to facilitate gene expression profiling of key genes involved in maintaining pluripotency and self-renewal of hPSC, and genes regulated during differentiation of hPSC. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Pluripotent Stem Cell qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ACTC1 |
CDX2 |
EN2 |
FOXA2 |
GFAP |
ISL1 |
MIXL1 |
NOG |
PECAM1 |
SOX1 |
TBX6 |
β-actin |
|
B |
AFP |
CER1 |
ENO2 |
FOXD3 |
GJA1 |
KLF4 |
MYF5 |
NPM1 |
POU5F1 |
SOX2 |
TCF2 |
GAPDH |
|
C |
ALB |
COL1A1 |
EOMES |
FOXJ3 |
GSC |
LEFTYA |
MYOD1 |
NR5A2 |
PTF1A |
SOX7 |
TCF3 |
LDHA |
|
D |
ALDH1A1 |
COL2A1 |
ESG1 |
GATA2 |
HAND1 |
LEFTYB |
NANOG |
OLIG2 |
REST |
SOX17 |
TDGF1 |
NONO |
|
E |
BRIX1 |
CXCR4 |
ESRRB |
GATA4 |
HDAC2 |
LIN28 |
NEUROD |
OTX2 |
REX1 |
T |
TERT |
PPIH |
|
F |
CD34 |
DES |
FGF4 |
GATA6 |
HLXB9 |
MEF2C |
NCAM1 |
PAX2 |
RUNX1 |
TAT |
TUBB3 |
GDC |
|
G |
CD90 |
DNMT3B |
FGF5 |
GBX2 |
HNF4A |
MEIS1 |
NES |
PAX6 |
RUNX2 |
TBX3 |
UTF1 |
PPC |
|
H |
CDH1 |
EMX2 |
FLT1 |
GDF3 |
IDH1 |
MESP1 |
NKX2.5 |
PDX-1 |
SALL4 |
TBX5 |
VIM |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK002 |
| 产地 | 美国 |
| 缩写 | GQH-hPSC |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK001 | 人类细胞凋亡qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK002 | 人类多能干细胞qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK003 | 人类细胞周期qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK005 | 人上皮细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK006 | 人类神经发育和再生qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK007 | 人体神经可塑性qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK008 | 人体神经传递和膜转运qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK009 | 人类星形胶质细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK010 | 人类黑素细胞发育和色素沉着qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK011 | 人类黑素细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK013 | 人类星形胶质细胞转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK017 | 人血管生成qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK018 | 人内皮细胞表型异质性qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK019 | 人内皮细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK020 | 人B细胞受体信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK021 | 人类T细胞受体信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK022 | 人内吞作用qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK023 | 人内皮细胞分化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK024 | 人体剪切应力和机械传导qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK025 | 人类Notch信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK026 | 人类Wnt信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK027 | 人类非经典Wnt信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK028 | 人类Wnt信号靶基因qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK029 | Human Tie2 / Angpt信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK034 | 人肺纤维化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK035 | 人体缺氧反应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK036 | 人类HIF1信号响应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK037 | 人巨噬细胞细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK038 | 人类吞噬作用qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + | |
| GK039 | 人类先天免疫反应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $298 | + |
