ScienCell's GeneQuery™ Human Cytoskeletal Remodeling qPCR Array Kit (GQH-CYT) profiles 88 key genes involved in cytoskeletal remodeling with a particular focus on Rho GTPase actin cytoskeleton remodeling. The cytoskeleton is responsible for maintaining cell shape, scaffolding, and regulating intracellular transport. It is also involved in numerous signaling pathways, cellular division, vesicular trafficking, and motility. Microfilaments, which are primarily actin polymers, are part of the cytoskeleton and regulated by the Rho family of GTPases. Brief examples of how included genes may be categorized are shown below:
- Cytoskeletal components: ACTA1, ACTB, ACTG1, ACTN1, MYH10, MYL1
- Integrins: ITGA1, ITGA3, ITGAV, ITGB1, ITGB3
- Extracellular matrix components: COL1A1, COL2A1, COL4A1, LAMA1, LAMB1
- Rho signaling molecules: CAV1, CDC42, RAC1, RHOA, ROCK1, ROCK2
- Transcription factors: CDX1, CDX2, SP1, TCF7, TCF7L1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Cytoskeletal Remodeling qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACTA1 |
ACTN3 |
CFL1 |
COL4A1 |
ITGA3 |
LAMB1 |
MYH2 |
MYL12B |
MYLK |
PTK2 |
SRC |
β-actin |
|
B |
ACTA2 |
ACTN4 |
CFL2 |
COL4A2 |
ITGA5 |
LAMC1 |
MYH3 |
MYL2 |
MYLK2 |
PXN |
TCF7 |
GAPDH |
|
C |
ACTB |
ACTR3B |
CLDN4 |
COL4A5 |
ITGA7 |
LEF1 |
MYH6 |
MYL3 |
MYLK3 |
RAC1 |
TCF7L1 |
LDHA |
|
D |
ACTC1 |
ARHGDIA |
CLDN5 |
FLNA |
ITGA8 |
LIMK1 |
MYH7 |
MYL4 |
MYLPF |
RHOA |
TCF7L2 |
NONO |
|
E |
ACTG1 |
CAV1 |
CLDN7 |
FN1 |
ITGAV |
MAPK1 |
MYH7B |
MYL5 |
NECTIN1 |
ROCK1 |
TJP1 |
PPIH |
|
F |
ACTG2 |
CDC42 |
COL1A1 |
GRB2 |
ITGB1 |
MAPK3 |
MYH8 |
MYL6 |
NKX2-1 |
ROCK2 |
TLN1 |
GDC |
|
G |
ACTN1 |
CDX1 |
COL1A2 |
ITGA1 |
ITGB3 |
MYH10 |
MYH9 |
MYL6B |
PAK1 |
SNAI1 |
TLN2 |
PPC |
|
H |
ACTN2 |
CDX2 |
COL2A1 |
ITGA2 |
LAMA1 |
MYH11 |
MYL1 |
MYL9 |
PIP5K1C |
SP1 |
VCL |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK104 |
| 产地 | 美国 |
| 缩写 | GQH-CYT |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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