Vascular endothelial cells lining the blood vessels actively participate in many vital biological processes. Studies have shown that mammary vascular endothelial cells (MVEC) secrete a mammaryderived growth inhibitor to modulate endothelial cell proliferation and differentiation of the mammary gland. MVEC also regulate trafficking of lymphocytes and the inflammatory response, and are thus implicated in mastitis. Further studies demonstrate a critical role for MVEC in breast cancer development through angiogenesis. Human MVEC (HMVEC) cultures are a great model for identifying targets for therapeutic development in breast cancer. HMVEC from ScienCell Research Laboratories are isolated from human breast. HMVEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMVEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HMVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMVEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HMVEC in vitro.