The adrenal gland plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. The secreted steroids arise from the three zones of the adrenal gland: the zona glomerulosa, zona fasciculate, and zona reticularis. Microvascular endothelial cells (MEC) line the blood vessels and contribute to many biological processes such as hemostasis, coagulation, trafficking of lymphocytes, and the inflammatory response. MEC regulate the secretion of adrenal hormones in the adrenal gland. Studies have shown that factors secreted from HAdMEC, such as endothelin-1, nitric oxide, adrenomedullin and proadrenomedullin NH2-terminal 20 peptide affect aldosterone secretion. HAdMEC, unlike brain and liver MECs, are highly resistant to glucocorticoid-induced cytotoxicity. HAdMEC from ScienCell Research Laboratories are isolated from human adrenal tissue. HAdMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HAdMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HAdMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdMEC are guaranteed to further culture for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HAdMEC in vitro.