Human synoviocytes (HS), the predominant cell type of healthy synovial tissue, are fibroblast-like cells. Synoviocytes form a distinct structure called the synovial lining layer. Synoviocytes produce synovial fluid, are responsible for absorption from the joint cavity and are in control of blood/synovial fluid exchanges. Synoviocytes proliferate, show anchorage-independent growth, and secrete a variety of effector molecules that promote inflammation and joint destruction. In addition, they are part of a complex network of autocrine and paracrine acting factors. Synoviocytes provide new insight into synovial tissue organization and morphogenesis and are a potential therapeutic target. HS from ScienCell Research Laboratories are isolated from human synovium. HS are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HS are characterized by fibroblast-like morphology and immunofluorescence with antibodies specific to CD 90 and/or fibronectin. HS are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HS are guaranteed for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Synoviocyte Medium (SM, Cat. #4701) for the culturing of HS in vitro.