The most abundant cell type in the bronchus is fibroblasts. Bronchial Fibroblasts (BF) display a mesenchymal stem cell phenotype and their principle function is to produce type III collagen, elastin, and proteoglycans of the extracellular matrix. Bronchial fibroblasts play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial for effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts can result in abnormal tissue function. For example, the excess fibroblast proliferation and collagen secretion that occurs from bronchial subepithelial fibrosis can result in airway obstruction and bronchial hyper-responsiveness. HBF from ScienCell Research Laboratories are isolated from human bronchus tissue. HBF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HBF are characterized by immunofluorescence with antibody specific to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HBF in vitro.