Meningeal cells surrounding the brain participate actively in the normal development of the central nervous system. They play important roles in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, laminating the cerebellar cortex. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex and dentate gyrus. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex in adult rat spinal cord lesion, promote axonal regrowth. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells. RMC from ScienCell Research Laboratories are isolated from CD® IGS rat leptomeningi. RMC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RMC are characterized by immunofluorescence; they are positive for fibronectin and negative for GFAP. RMC are negative for mycoplasma, bacteria, yeast and fungi. RMC are guaranteed to further expand for 5 population doublings under the conditions specified by ScienCell Research Laboratories. Recommended Medium It is recommended to use Meningeal Cell Medium (MenCM, Cat. #1401) for the culturing of RMC in vitro.