ScienCell's GeneQuery™ Human Cell Growth and Division qPCR Array Kit (GQH-CGD) is designed to facilitate gene expression profiling of 88 key genes involved in cell growth and division. Tight control of these processes is essential to ensure that the hereditary information is correctly passed on from parent cells to daughter cells, and that cell growth is strictly regulated. Failure of such control gives rise to mutations and/or tumorigenesis. Brief examples of how included genes may be grouped are shown below:
- Cell cycle regulation: ATM, CCNA2, CCNB1, CCNE2, CDC20, CDC25C, CDK1, CDK2, CHEK1, NBN, RAD1, RAD17
- Mitosis: APC/C complex, CDC genes, BUB1, BUB1B, EGF, EREG, KNTC1, NPM2, MAD2L1, MAD2L2, PKMYT1, TGFA, TGFB1, TTK
- Meiosis: APC/C complex, CDC genes, RAD51, RBX1, SKP1, SMC1A, SMC3
- Spindle checkpoint: AURKA, BIRC5, BUB1, CDC16, CDC27, DCTN1, KNTC1, PAFAH1B1, SMC1A, SMC3, STMN1, TTK
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Cell Growth and Division qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ADCY1 |
ANAPC2 |
BUB1 |
CCNE2 |
CDC27 |
EGF |
KNTC1 |
NBN |
PPP3CA |
RAD17 |
TARDBP |
β-actin |
|
B |
ADCY2 |
ANAPC4 |
BUB1B |
CD28 |
CDK1 |
EREG |
MAD2L1 |
NPM2 |
PPP3R1 |
RAD51 |
TGFA |
GAPDH |
|
C |
ADCY3 |
ANAPC5 |
CALM1 |
CDC16 |
CDK2 |
ESPL1 |
MAD2L2 |
PAFAH1B1 |
PRKACA |
RBX1 |
TGFB1 |
LDHA |
|
D |
ADCY8 |
ANAPC7 |
CAMK2A |
CDC20 |
CDKN2B |
FBXW11 |
MAP2K1 |
PBRM1 |
PRKACB |
RPS6KA1 |
TTK |
NONO |
|
E |
ANAPC1 |
ATM |
CAMK2G |
CDC23 |
CHEK1 |
IGF1 |
MAPK1 |
PKMYT1 |
PRKACG |
SKP1 |
TTN |
PPIH |
|
F |
ANAPC10 |
AURKA |
CCNA2 |
CDC25B |
CHP2 |
IGF1R |
MAPK12 |
PLK1 |
PRKX |
SMC1A |
YWHAB |
GDC |
|
G |
ANAPC11 |
BIRC5 |
CCNB1 |
CDC25C |
CUL1 |
ITPR1 |
MAPK3 |
PPP1CA |
PTTG1 |
SMC3 |
YWHAE |
PPC |
|
H |
ANAPC13 |
BTRC |
CCNB2 |
CDC26 |
DCTN1 |
ITPR2 |
MRE11A |
PPP2CA |
RAD1 |
STMN1 |
YWHAG |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK062 |
| 产地 | 美国 |
| 缩写 | GQH-CDG |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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