ScienCell’s One-Step GoldNStart TaqGreen RT-qPCR master mix, 4x (GTGRTMM) is designed for sensitive RNA reverse transcription and quantification by SYBR® Green dye-based reverse transcription quantitative PCR (RT-qPCR). The ready-to-use 4x master mix contains UNG/dUTP (to eliminate PCR carryover contamination), engineered M-MLV reverse transcriptase with “tepid-start” property, Taq DNA polymerase with “hot-start” property, dNTPs, a buffer system for robust reverse transcription and qPCR, and an inert gold-color loading indicator (ScienCell, catalog #GQ300G). The “tepid-start” and “hot-start” property achieved through ScienCell’s unique chemically modified M-MLV reverse transcriptase and Taq DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert gold-color loading indicator allows for better visualization and tracking of sample loading in qPCR plates or tubes.