ScienCell's GeneQuery™ Human Endothelin Signaling qPCR Array Kit (GQH-EDN) surveys 88 key genes involved in the endothelin signaling transduction with a particular focus on endothelin-1. Endothelins are secreted proteins that regulate vasoconstriction and are produced primarily in the endothelium. Endothelin receptors are also present in cells of the central nervous system and abnormal signaling may be involved in seizures and peripheral neuropathy. Other diseases implicated in aberrant endothelin signaling include cardiovascular disorders, vasospasm, cardiac hypertrophy, and Dengue fever. Brief examples of how included genes may be categorized are shown below:
- Calcium regulation: CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D
- Cytoskeletal remodeling: CAPN2, MAP2K1, PLD1, PLD2, RHOA
- Cell cycling regulation: CCND3, CDC42, ITPR1, ITPR3, RIPK1
- Transcription factors: ELK1, FOS, JUN, MYC, SP1
- RAS superfamily GTPases: HRAS, KRAS, NRAS, RAC1, RAP1A
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Endothelin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ADCY1 |
CACNA1D |
CDC42 |
EDNRA |
GSK3B |
MAP2K1 |
MYC |
PIK3R1 |
PPP1CA |
PTGS2 |
RIPK1 |
β-actin |
|
B |
ADCY3 |
CALM1 |
CGA |
EDNRB |
HBEGF |
MAP3K1 |
NOS3 |
PIK3R2 |
PPP3CC |
PTK2B |
SHC1 |
GAPDH |
|
C |
ADCY8 |
CALM2 |
CRK |
EGFR |
HRAS |
MAPK13 |
NPPB |
PIK3R3 |
PRKCA |
RAC1 |
SOS1 |
LDHA |
|
D |
AKT1 |
CAMK2A |
DGKB |
ELK1 |
ITPR1 |
MAPK14 |
NRAS |
PLA2G4A |
PRKCD |
RAF1 |
SOS2 |
NONO |
|
E |
AKT2 |
CAMK2B |
DGKE |
FOS |
ITPR2 |
MAPK3 |
NTRK1 |
PLA2G7 |
PRKCE |
RAP1A |
SP1 |
PPIH |
|
F |
ASIC1 |
CAMK2D |
ECE1 |
GNAI1 |
ITPR3 |
MAPK9 |
PIK3CA |
PLCG1 |
PTGER2 |
RAPGEF1 |
SRC |
GDC |
|
G |
ATF4 |
CAPN2 |
EDN1 |
GNAQ |
JUN |
MMP2 |
PIK3CB |
PLD1 |
PTGER4 |
RASGRP1 |
STAT5A |
PPC |
|
H |
CACNA1C |
CCND3 |
EDN3 |
GRB2 |
KRAS |
MT-CO2 |
PIK3CD |
PLD2 |
PTGS1 |
RHOA |
TRPV1 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK107 |
| 产地 | 美国 |
| 缩写 | GQH-EDN |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK040 | 人成纤维细胞生长因子信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK041 | 人体皮肤伤口愈合qPCR检测套件 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK056 | 人体止血qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK060 | 人类昼夜节律qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK061 | 人类DNA损伤感应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK062 | 人类细胞生长和分裂qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK063 | 人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK069 | 人类小胶质细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK072 | 人类适应性免疫反应qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK073 | 人类小胶质细胞Sensome qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK074 | 人角质细胞生物学qPCR检测 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK078 | 人类神经细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK079 | 人类软骨细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK080 | 人成骨分化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK081 | 人类周细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK084 | 人体轴突导向qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK087 | 人类染色质组织和重塑qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK088 | 人VEGF信号通路qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK089 | 人类Ephrin信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK090 | 人体细胞连接qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK091 | 人肝星状细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK092 | 人胰腺星状细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK093 | Human Renal Mesangial Cell Biology qPCR Array Kit | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK094 | Human Mesenchymal Stem Cell Differentiation qPCR Array | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK096 | 人类雪旺细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK097 | 人类平滑肌细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK098 | 人类心脏肌细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK099 | 人类骨骼肌细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK100 | 人角质形成细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
