ScienCell's GeneQuery™ Human Skeletal Muscle Cell Biology qPCR Array Kit (GQH-SKM) is designed to facilitate gene expression profiling of 88 key genes involved in human skeletal muscle cell biology, including skeletal muscle contractility, development, proliferation, and differentiation. Genes implicated in selected skeletal muscular disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

• Skeletal muscle cell markers: SGCA, CKM, EIF5A, ENO2, FABP3, MSTN, ITGA7, ITGB1, MCAM, MYOD1, MYOG, NCAM1, TNNI1

• Skeletal muscle contractility: ACTA1, TTN, TCAP, MYH3, TNNT3, SLC2A1/4, TPM2, CPT1B, CPT2, PDHA1/2, PDHB, CACNA1F, DMD

• Skeletal muscle cell development and proliferation: MYH3/8, ACTA1, PAX3/7, SIX1/4, MYF5/6, RPS6KB1, PITX2, BCL2, ZNF513, MYOD1, MYOG, EYA1/2
• Skeletal muscle cell differentiation: MYOD1, MYF5/6, MYOG, TCF12, TRIM28, MEF2A/C,  HDAC1, EZH2, SRF, JUNB, FOXO3
• Genes implicated in
• muscular atrophy: AR, MTM1, ACTA1, TPM2, MYH2/7, CRYAB, GNE, DES, RYR1
• muscular dystrophy: DMD, BEST1, UTRN, LTBP4, OTC, GK, LMNA, EMD, FHL1, SYNE2, TMEM43

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Skeletal Muscle Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development