ScienCell's GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Kit (GQH-TEK) is designed to facilitate gene expression profiling of 88 key genes involved in Tie2 signaling transduction, which is a key component in the formation of blood vessels during angiogenesis. Brief examples of how included genes may be grouped according to function are shown below:
- Ligands & receptors: ANGPT1, ANGPT2, TEK, TIE1
- Morphology: GRB2, PTPN11, SOS1, PTK2, RHOA
- Motility: PLD1, MAP2K1, DOK2, NCK1, CRK
- Transcription factors: STAT1, STAT5B, MEF2A, FOXO3, EGR1
- Cell cycle regulation: CDC42, NFKB1, TNIP2, RIPK1, DIABLO
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ADSS |
CDC42 |
DOK2 |
FOXO3 |
HIF1A |
ITGB5 |
MMP2 |
PDIA3 |
PTPN11 |
SELE |
TEK |
β-actin |
|
B |
AKT1 |
CDKN1A |
DOK4 |
FYN |
HRAS |
KLF2 |
MTOR |
PGK1 |
PXN |
SELP |
TIAM1 |
GAPDH |
|
C |
AKT2 |
CRK |
EGR1 |
GJA5 |
ICAM1 |
MAP2K1 |
NCK1 |
PIK3CA |
RAC1 |
SOS1 |
TIE1 |
LDHA |
|
D |
ALDOA |
CTNNB1 |
ELF2 |
GRB14 |
ILK |
MAPK1 |
NFKB1 |
PIK3R1 |
RAF1 |
SPHK1 |
TLN1 |
NONO |
|
E |
ANGPT1 |
CXCL12 |
ELK1 |
GRB2 |
ITGA5 |
MAPK14 |
NOS3 |
PIK3R2 |
RASA1 |
STAT1 |
TNIP2 |
PPIH |
|
F |
ANGPT2 |
DIABLO |
EPAS1 |
GRB7 |
ITGAV |
MAPK3 |
NOX1 |
PLCG1 |
RELA |
STAT3 |
TXNRD1 |
GDC |
|
G |
ARHGAP5 |
DIAPH1 |
FES |
GYS1 |
ITGB1 |
MAPK7 |
NR4A1 |
PLD1 |
RHOA |
STAT5A |
VCAM1 |
PPC |
|
H |
BIRC5 |
DLL4 |
FOXO1 |
HAGH |
ITGB3 |
MEF2A |
PAK1 |
PTK2 |
RIPK1 |
STAT5B |
VEGFA |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK029 |
| 产地 | 美国 |
| 缩写 | GQH-TEK |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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