ScienCell's GeneQuery™ Human Astrocyte Transcription Factors qPCR array kit (GQH-ATF) is designed to facilitate gene expression profiling of some key transcription factors invloved in human astrocyte differentiation, maintenance, function, and signaling pathways. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Astrocyte Transcription Factors qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ARID3B |
DPF3 |
FLI1 |
HEY1 |
JUN |
LIN28B |
OTX1 |
PRRX1 |
SORBS2 |
ZBTB46 |
ZNF138 |
β-actin |
|
B |
BCL6 |
E2F2 |
FOS |
HOPX |
KAT6A |
MBNL1 |
PBX2 |
RAPGEF5 |
SOX3 |
ZC3HAV1 |
ZNF219 |
GAPDH |
|
C |
BHLHE40 |
EBF1 |
FOXG1 |
ID1 |
KLF5 |
MYCN |
PHF11 |
RBM20 |
SUV420H1 |
ZFHX3 |
ZNF385D |
LDHA |
|
D |
CBX2 |
EGR1 |
GLI2 |
IRF9 |
KLF6 |
NFE2L3 |
PKNOX2 |
RCOR2 |
TRIT1 |
ZFP14 |
ZNF431 |
NONO |
|
E |
CHD7 |
EMX2 |
GLIPR1 |
IRX2 |
KLF9 |
NFIA |
PLAGL2 |
RUNX2 |
ZBTB20 |
ZFP3 |
ZNF443 |
PPIH |
|
F |
CSDC2 |
ETS2 |
GLIS3 |
IRX5 |
KNTC1 |
NFIB |
POU3F2 |
SALL2 |
ZBTB34 |
ZFP36 |
ZNF462 |
GDC |
|
G |
CXXC4 |
ETV4 |
GTF2F2 |
JADE2 |
LHX2 |
NFIX |
PRDM16 |
SALL4 |
ZBTB39 |
ZMYND8 |
ZNF679 |
PPC |
|
H |
CXXC5 |
EZH2 |
HES6 |
JADE3 |
LIN28 |
NR2E1 |
PRDM8 |
SMARCA2 |
ZBTB4 |
ZNF135 |
ZNF696 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK013 |
| 产地 | 美国 |
| 缩写 | GQH-ATF |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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