联系我们
订购指南
购物车(0)
铁检测试剂盒
Iron Assay
品牌:
ScienCell
货号:
8448
价格:
$305
产品说明书下载:
产品预定
>
产品预定,提交订单信息之后,我们会第一时间与您取得联系
{{item}}
Iron is an element that plays an essential role in a number of biological processes. Iron is bound to several proteins such as the oxygen-transporting hemoglobin in the red blood cells and oxygen storage protein myoglobin in the muscle. It is also stored by ferritin and hemosiderin. Inorganic iron contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase. In this colorimetric assay, ferric carrier protein will dissociate ferric into solution under acidic conditions. After they are reduced to the ferrous ions, these ions react with chromogen to produce a stable colored complex and give absorbance at 590 nm. The color intensity thus formed is directly proportional to the concentration of iron in the sample. A specific chelate chemical is included in the buffer to block copper ion interference.
货号8448
产地美国
缩写IR
规格 100 tests
用途科研
储存-20度
运输干冰
No Publication available at this time

Question:

Hello, I wanted some clarification for part B: preparation of test samples for the Iron Assay Kit (Catalog #8448). I am confused what it means by " 4 volumes of the assay buffer", and "93 volumes of assay buffer (8448a), 2 volumes reductant (8448e) and 5 volumes sample reagents (8448d)." Does volumes just mean any unit of volume as long as that ratio is used? Thank you, Lauren Nardacci

Answers:

  • Posted by Manoj Sharma on Wednesday, January 6, 2016

    Hi Lauren,

    We have revised the preparation as follows:

    B. Preparation of test samples
    1. One volume of cells or tissues can be homogenized in 4 volumes of the assay buffer (8448a). Centrifuge the samples at 13,000 ×g for 10 minutes to remove insoluble material.
    2. Prepare a fresh working buffer by mixing assay buffer (8448a), reductant (8448e) and sample reagents (8448d) at 93:2:5 ratio (volume ratio). (e.g., 930 μL mixing assay buffer, 20 μL reductant, and 50 μL sample reagents)
    3. Mix sample (cell lysate or serum) with equal volume of working buffer.
    4. Centrifuge the mixed sample 13,000 ×g for 5 minutes and take the supernatant as the test samples for the iron assay.
    5. Test samples should be serial diluted to make sure the readings are within the standard curve range. Prepare test samples to a final volume of 50 μL/well on the 96-well flat bottom plate.

细胞检测试剂盒
细胞检测试剂盒
货号 产品名称 规格 价格 指令
0223 茜素红S染色试剂盒 ARed 100ml $86 +
0833 人胚层鉴定试剂盒 HGL PCR 50 reactions $374 +
0843 油红O染色试剂盒 OEed 100 ml/bottle - 2 bottles $110 +
0853 人干细胞多能性检测试剂盒  HSC PCR 50 reactions $374 +
3D-4110 3D肾小管上皮细胞形成试剂盒 3D-RTF Kit $1054 +
8008 I型胶原细胞粘附检测试剂盒 48 tests $273 +
8018 纤维粘连细胞粘附检测试剂盒 48 tests $273 +
8028 MTT细胞活力和增殖检测试剂盒 1000 tests in 96-well plate $160 +
8038 WST-1细胞活力和增值检测试剂盒 1000 tests in 96-well plate $273 +
8058 细胞衰老检测试剂盒 50 tests/35 mm plate $273 +
8068 β-半乳糖苷酶比色检测试剂盒 GalC 50 tests/35 mm plate $273 +
8078 LDH细胞毒性检测试剂盒 LDH 500 tests $226 +
8088 TUNEL色标记细胞凋亡检测试剂盒 50 tests $477 +
8098 一氧化氮比色法检测试剂盒 NO 250 tests $316 +
8108 磷酸酶检测试剂盒 pNPP 500 tests $137 +
8118 孔雀绿磷酸盐检测试剂盒 MGPA 2500 tests $137 +
8128 比色钙法分析试剂盒 CCA 250 tests $240 +
8138 活/死细胞染色试剂盒 1000 tests $354 +
8148 比色法GAPDH分析试剂盒 GAPDH 100 tests $149 +
8158 体外管腔形成分析试剂盒 50 tests $348 +
8168 总抗氧化能力检测试剂盒 TACA 100 tests $297 +
8178 胶原 I-3D凝胶试剂盒 100 mg $187 +
8198 超氧化物歧化酶分析试剂盒 SOD 100 tests $137 +
8198 超氧化物歧化酶分析试剂盒 SOD 100 tests $137 +
8208 支原体PCR检测试剂盒 100 Tests $292 +
8218 过氧化氢酶活性分析试剂盒 CAT 100 tests $273 +
8218 过氧化氢酶活性分析试剂盒 CAT 100 tests $273 +
8228 细胞凋亡蛋白酶-3分析试剂盒 100 tests $354 +
8238 谷胱甘肽过氧化氢酶检测试剂盒 GPx 100 tests $374 +
8238 谷胱甘肽过氧化氢酶检测试剂盒 GPx 100 tests $374 +
合作品牌
公司信息
代理品牌
联系我们
电话:
+86-10-63804220
+86-10-63803739
+86-10-63861816
地址:北京市丰台区丰体北路5号国丰大厦122室
传真:010-63822556
邮箱:beijingyuhengfeng@163.com
京公网安备 11010602006112号
版权所有: 北京裕恒丰科技有限公司 Copyright 2007-2021 金鹰品牌设计技术支持
京公网安备 11010602006112号 您是第 10954517 位访问裕恒丰
+86 10 6380 4220
+86 10 6380 3739
+86 10 6386 1816