联系我们
订购指南
购物车(0)
人端粒长度和线粒体DNA拷贝数双重分析定量qPCR分析试剂盒(绝对定量)AHDQ
Absolute Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification qPCR Assay Kit
品牌:
ScienCell
货号:
8958
价格:
$556
产品说明书下载:
产品预定
>
产品预定,提交订单信息之后,我们会第一时间与您取得联系
{{item}}
Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions and oncogenesis. Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in the energy production. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Evidence suggests that telomere length and mitochondrial dysfunction are positively correlated in cellular aging and other age-related disorders such as cancer, diabetes and neurodegenerative diseases. A possible mechanistic link between them has been proposed through the PPAR signaling pathway. However, the specifics of the mechanism still has to be elucidated. ScienCell's Absolute Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification qPCR Assay Kit (AHDQ) is designed to simultaneously quantify the average telomere length and mtDNA copy number of a human cell population using qPCR. The telomere primer set recognizes and amplifies telomere sequences. The mtDNA primer set recognizes and amplifies one of the most conserved regions on human mtDNA and will not amplify any off-target sequence on nuclear genomic DNA. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on human chromosome 17, and serves as reference for data normalization. The reference genomic DNA sample with known telomere length and mtDNA copy number serves as a reference for calculating the telomere length and the mtDNA copy number of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.
货号8958
产地美国
缩写AHDQ
规格100 reactions
用途科研
储存-20度
运输干冰

Publications Promotion

Has your paper been published using one or more of Sciencell products? Get rewarded! Receive 10% off one order*. See details >>

 

1.) Martin LF, Richardson LS, da Silva MG, Sheller- Miller S, Menon R. (2019) Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway. Biology of Reproduction.

2.) Babu H, Ambikan AT, Gabriel EE, Akusjarvi SS, Mupanni NR, Sperk M, Cheedarla N, Palaniapan AN, Sridhar R, Sundaraj V, Tripathy SP, Nowak P, Hanna LE, Neogi U. (2018) Systemic inflammation following long-term successful antiretroviral therapy in people living with HIV (PLHIV). bioRxiv preprint.

3.) Iezzi I, Cerqueni G, Licini c, Lucarini G, Belmonte M. (2018) Dental pulp stem cells senescence and regenerative potential relationship. Journal of Cellular Physiology. 234(5):7186-7197 

4.) Lazzarini R, Nicolai M, Pirani V, Mariorri C, DiPrimio R. (2018) Effects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells. Aging. 10(11):3173-3184

5.) Shirato K, Takanari J, Koda T, Sakurai T, Ogasawara J, Ohno H, Kizaki T. (2018) A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study. Environmental Health and Preventative Medicine. 23:40
There are no product questions yet.
细胞检测试剂盒
细胞检测试剂盒
货号 产品名称 规格 价格 指令
8258 碱性磷酸酶活力检测试剂盒 ALP 500 tests $380 +
8268 线粒体分离试剂盒 MITOISO 100 tests $321 +
8278 细胞色素C氧化酶检测试剂盒 COX 100 tests $347 +
8278 细胞色素C氧化酶检测试剂盒 COX 100 tests $347 +
8288 碱性磷酸酶染色试剂盒(红) ALPr 500 tests $432 +
8298 人间充质干细胞脂肪形成检测试剂盒 HMSC-A PCR 50 reactions $311 +
8308 L-乳酸盐检测试剂盒 LAC 100 tests $141 +
8318 柠檬酸合成酶检测试剂盒 CS 50 tests in cuvette or 250 tests in 96-well plate $458 +
8318 柠檬酸合成酶检测试剂盒 CS 50 tests in cuvette or 250 tests in 96-well plate $458 +
8328 人间充质干细胞软骨形成检测试剂盒 HMSC-C PCR 50 reactions $311 +
8338 谷氨酸检测试剂盒 GLU 100 tests $399 +
8358 谷氨酸脱氢酶检测试剂盒 GDH 100 tests $399 +
8368 NAD/NADH检测试剂盒 NAD 100 tests $401 +
8368 NAD/NADH检测试剂盒 NAD 100 tests $401 +
8378 阿尔新蓝染色试剂盒 ABlue 100 tests $117 +
8388 储液瓶 传统风格 125ml 45mm颈尺寸 带盖 灭菌 100 tests $354 +
8398 葡萄糖6磷酸酶检测试剂盒 G6P 100 tests $256 +
8408 已糖激酶检测试剂盒 HK 100 tests $319 +
8418 葡萄糖检测试剂盒 GL 100 tests $284 +
8428 葡糖6磷酸脱氢酶检测试剂盒  G6PDH 100 tests $256 +
8438 尿酸检测试剂盒 US 100 tests $374 +
8448 铁检测试剂盒 100 tests $305 +
8458 黄嘌呤氧化酶检测试剂盒 XO 100 tests $138 +
8458 黄嘌呤氧化酶检测试剂盒 XO 100 tests $138 +
8468 甘油检测试剂盒 GLY 100 tests $222 +
8478 丙氨酸转氨酶检测试剂盒 ALT 100 tests $297 +
8498 甘油三酸酯检测试剂盒 TG 100 tests $285 +
8508 3-甘油磷酸脱氢酶检测试剂盒  GPDH 100 tests $423 +
8518 总谷胱甘肽检测试剂盒 TGA 100 tests $258 +
8518 总谷胱甘肽检测试剂盒 TGA 100 tests $258 +
合作品牌
公司信息
代理品牌
联系我们
电话:
+86-10-63804220
+86-10-63803739
+86-10-63861816
地址:北京市丰台区丰体北路5号国丰大厦122室
传真:010-63822556
邮箱:beijingyuhengfeng@163.com
京公网安备 11010602006112号
版权所有: 北京裕恒丰科技有限公司 Copyright 2007-2021 金鹰品牌设计技术支持
京公网安备 11010602006112号 您是第 10954517 位访问裕恒丰
+86 10 6380 4220
+86 10 6380 3739
+86 10 6386 1816