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人线粒体DNA拷贝数定量qPCR分析试剂盒(绝对定量)AHMQ
Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit
品牌:
ScienCell
货号:
8948
价格:
$370
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Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in the energy production. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number has been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases.

ScienCell's Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (AHMQ) is designed to quantify the average mtDNA copy number of a human cell population. The mtDNA primer set recognizes and amplifies one of the most conserved regions on human mtDNA and will not amplify any off-target sequence on nuclear genomic DNA. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on human chromosome 17 and serves as reference for data normalization. The reference genomic DNA sample with known mtDNA copy number serves as a reference for calculating the mtDNA copy number of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.

货号8948
产地美国
缩写AHMQ
规格100 reactions
用途科研
储存-20度
运输干冰

Publications Promotion

Has your paper been published using one or more of Sciencell products? Get rewarded! Receive 10% off one order*. See details >>

 

1.) Martin LF, Richardson LS, da Silva MG, Sheller- Miller S, Menon R. (2019) Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway. Biology of Reproduction.

2.) Babu H, Ambikan AT, Gabriel EE, Akusjarvi SS, Mupanni NR, Sperk M, Cheedarla N, Palaniapan AN, Sridhar R, Sundaraj V, Tripathy SP, Nowak P, Hanna LE, Neogi U. (2018) Systemic inflammation following long-term successful antiretroviral therapy in people living with HIV (PLHIV). bioRxiv preprint.

3.) Iezzi I, Cerqueni G, Licini c, Lucarini G, Belmonte M. (2018) Dental pulp stem cells senescence and regenerative potential relationship. Journal of Cellular Physiology. 234(5):7186-7197 

4.) Lazzarini R, Nicolai M, Pirani V, Mariorri C, DiPrimio R. (2018) Effects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells. Aging. 10(11):3173-3184

5.) Shirato K, Takanari J, Koda T, Sakurai T, Ogasawara J, Ohno H, Kizaki T. (2018) A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study. Environmental Health and Preventative Medicine. 23:40
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