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小鼠胚胎成纤维细胞--丝裂霉素处理
Mouse Embryonic Fibroblasts-mitomycin C treated from CF1
品牌:
ScienCell
货号:
M7570-5-mt
价格:
$105
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Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells. MEF-mt from ScienCell Research Laboratories are isolated from embryonic day 14 CF-1 mouse embryos. These cells have been treated with mitomycin C to prevent further cell division. They are cryopreserved at passage 3 and delivered frozen. Each vial contains 1 x 106 cells in 1 ml volume. MEF-mt are characterized by immunofluorescence with antibody specific to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast, and fungi. MEF are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MEF-mt are not recommended for expanding as mitomycin C treatment prevents further cell proliferation. Recommended Medium It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF-mt in vitro.
货号M7570-5-mt
产地北美
缩写MEF-mt
规格5 x 10^6 cells/vial
用途科研
储存液氮
运输干冰
小鼠细胞系统
小鼠细胞系统
货号 产品名称 规格 价格 指令
M1200 小鼠脑血管周细胞 5 x 10^5 cells/vial $608 +
M1200-57 小鼠脑血管周细胞 5 x 10^5 cells/vial $633 +
M1400 小鼠脑膜细胞 5 x 10^5 cells/vial $446 +
M1400-57 小鼠脑膜细胞 5 x 10^5 cells/vial $489 +
M1510 小鼠脑脊神经元 1 x 10^6 cells/vial $477 +
M1510-57 小鼠脑脊神经元 1 x 10^6 cells/vial $531 +
M1520 小鼠皮质神经元 1 x 10^6 cells/vial $477 +
M1520-57 小鼠皮质神经元 1 x 10^6 cells/vial $531 +
M1530 小鼠颗粒细胞 1 x 10^6 cells/vial $477 +
M1530-57 小鼠颗粒细胞 1 x 10^6 cells/vial $519 +
M1540 小鼠海马神经元 1 x 10^6 cells/vial $477 +
M1540-57 小鼠海马趾神经元 1 x 10^6 cells/vial $531 +
M1550 小鼠黑质神经元 1 x 10^6 cells/vial $477 +
M1550-57 小鼠黑质神经元 1 x 10^6 cells/vial $489 +
M1560 小鼠纹状体神经元 1 x 10^6 cells/vial $477 +
M1570 小鼠腹脊髓神经元 1 x 10^6 cells/vial $477 +
M1580 小鼠背脊髓神经元 1 x 10^6 cells/vial $477 +
M1580-57 小鼠背脊髓神经元 1 x 10^6 cells/vial $531 +
M1590 小鼠脊髓神经元 1 x 10^6 cells/vial $477 +
M1590-57 小鼠腹脊髓神经元 1 x 10^6 cells/vial $531 +
M1700 小鼠雪旺细胞 5 x 10^5 cells/vial $477 +
M1700-57 小鼠雪旺细胞 5 x 10^5 cells/vial $519 +
M1710 小鼠神经束膜细胞 5 x 10^5 cells/vial $446 +
M1710-57 小鼠神经束膜细胞 5 x 10^5 cells/vial $489 +
M1800 小鼠星形胶质细胞 5 x 10^5 cells/vial $446 +
M1800-57 小鼠星形胶质细胞 5 x 10^5 cells/vial $489 +
M1810 小鼠小脑星形胶质细胞 5 x 10^5 cells/vial $446 +
M1810-57 小鼠小脑星形胶质细胞 5 x 10^5 cells/vial $489 +
M1820 小鼠海马趾星形胶质细胞 5 x 10^5 cells/vial $446 +
M1820-57 小鼠海马趾星形胶质细胞 5 x 10^5 cells/vial $489 +
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