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Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.
MEF from ScienCell Research Laboratories are isolated from embryonic day 14 CF-1 mouse embryos. These cells are cryopreserved at P0 and delivered frozen. Each vial contains 1 x 106 cells in 1 ml volume. MEF are characterized by immunofluorescence with antibody specific to fibronectin. MEF are negative for mycoplasma, bacteria, yeast, and fungi. MEF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS,
Cat. #0010, 0025, 0500) for culturing MEF in vitro.
货号 | M7570-5 |
产地 | 北美 |
缩写 | MEF |
规格 | 5 x 10^6 cells/vial |
用途 | 科研 |
储存 | 液氮 |
运输 | 干冰 |
小鼠细胞系统
小鼠细胞系统
货号 | 产品名称 | 规格 | 价格 | 指令 | |
M1830 | 小鼠脊髓星形胶质细胞 | 5 x 10^5 cells/vial | $446 | + | |
M1830-57 | 小鼠脊髓星形胶质细胞 | 5 x 10^5 cells/vial | $489 | + | |
M1840 | 小鼠脑干星形胶质细胞 | 5 x 10^5 cells/vial | $446 | + | |
M1850 | 小鼠中脑星形胶质细胞 | 5 x 10^5 cells/vial | $446 | + | |
M1860-57 | 小鼠星形胶质细胞-成鼠 | 5 x 10^5 cells/vial | $489 | + | |
M1900 | 小鼠小胶质细胞 | 1 x 10^6 cells/vial | $608 | + | |
M1900-57 | 小鼠小胶质细胞 | 1 x 10^6 cells/vial | $714 | + | |
M1920 | 小鼠巨噬细胞 | 1 x 10^6 cells/vial | $372 | + | |
M1920-10 | 小鼠巨噬细胞 | 1 x 10^7 cells/vial | $1483 | + | |
M1920-2 | 小鼠巨噬细胞 | 2 x 10^6 cells/vial | $588 | + | |
M1920-5 | 小鼠巨噬细胞 | 5 x 10^6 cells/vial | $932 | + | |
M1920-57 | 小鼠巨噬细胞 | 1 x 10^6 cells/vial | $417 | + | |
M1920-57-2 | 小鼠巨噬细胞 | 2 x 10^6 cells/vial | $649 | + | |
M1930 | 小鼠骨髓单核细胞 | 10 x 10^6 cells/vial | $322 | + | |
M1930-25 | 小鼠骨髓单核细胞 | 25 x 10^6 cells/vial | $632 | + | |
M1930-57 | 小鼠骨髓单核细胞 | 10 x 10^6 cells/vial | $365 | + | |
M2300-57 | 小鼠真皮成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M2530 | 小鼠淋巴成纤维细胞 | 5 x 10^5 cells/vial | $446 | + | |
M2530-57 | 小鼠淋巴成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M2540 | 小鼠淋巴单核细胞 | 10 x 10^6 cells/vial | $322 | + | |
M2540-57 | 小鼠淋巴单核细胞 | 10 x 10^6 cells/vial | $365 | + | |
M2670-57 | 小鼠唾腺成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M3040 | 小鼠肺泡巨噬细胞 | 1 x 10^6 cells/vial | $358 | + | |
M3040-57 | 小鼠肺泡巨噬细胞 | 1 x 10^6 cells/vial | $413 | + | |
M3200 | 小鼠肺泡上皮细胞 | 1 x 10^6 cells/vial | $495 | + | |
M3200-57 | 小鼠肺泡上皮细胞 | 1 x 10^6 cells/vial | $550 | + | |
M3300 | 小鼠肺成纤维细胞 | 5 x 10^5 cells/vial | $446 | + | |
M3300-57 | 小鼠肺成纤维细胞 | 5 x 10^5 cells/vial | $477 | + | |
M4100 | 小鼠肾小管上皮细胞 | 5 x 10^5 cells/vial | $446 | + | |
M4100-57 | 小鼠肾小管上皮细胞 | 5 x 10^5 cells/vial | $489 | + |