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内皮细胞培养基 ECM
Endothelial Cell Medium
品牌:
ScienCell
货号:
1001
价格:
$139
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内皮细胞培养基是为正常人类微血管内皮细胞体外培养设计的适于其生长的完全培养基。是经灭菌的液体培养基,包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(5%)。该培养基缓冲体系为重碳酸盐,在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长,并为其达到理想营养平衡状态提供数量上和质量上的保证。


内皮细胞培养基包含500 ml基础培养基,25ml胎牛血清(FBS,目录编号0025),5ml内皮细胞生长添加物(ECGS,目录编号1052),和5 ml青霉素/链霉素溶液(P/S,目录编号0503)


货号1001
产地北美
缩写ECM
规格500ml
用途科研
储存4度,-20度
运输胶冰
1.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) 'A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation.'

2.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) 'In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications.'

3.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) 'Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60.'

4.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) 'Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis.'

5.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) 'Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction.'

6.) Voigt J, Christensen J, Shastri VP. (2014) 'Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae.'

7.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) 'Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization.'

8.) Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MBK. (2014) 'Ethyl-p-methoxycinnamate isolated from kaempferiagalanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-?, and angiogenesis by blocking endothelial functions.'

9.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) 'The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus.'

10.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) 'P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models.'

11.) Lim PJ, Chu JJH. (2014) 'A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells.'

12.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) 'The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells.'

13.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) 'Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection.'

14.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) 'Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains.'

15.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) "Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection."?Int j med sci. 11: 538.

16.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) "Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains."?Infect immune. 82: 949-59.

17.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.

18.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells."?PLoSnegl trop dis. 8: e2661.

19.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) "P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models."?PloS one. 9: e102442.

20.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) "The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus."?Cardiovascdiabetol. 13: 82.

21.) Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MBK. (2014) "Ethyl-p-methoxycinnamate isolated from kaempferiagalanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-&aplha;, and angiogenesis by blocking endothelial functions."?Clinics. 69: 134-44.

22.) Voigt J, Christensen J, Shastri VP. (2014) "Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae."?ProcNatlAcad Sci. 111: 2942-47.

23.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) "Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization."?BMC biotechnol. 14: 17.

24.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) "Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction."?Oxid med cell longev. 2014: 904834.

25.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) "Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis."?PloS one. 9: e88243.

26.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) "Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60."?Cell molimmunol. 11: 285-93.

27.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) "In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications."?PloS one. 9: e98674.

28.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) "A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation."?PloS one. 9: e89634.

29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.

30.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box."?BMC Neurosci. 14: 18.

31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242

32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.

33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.

34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.

35.) Zhao XC, Dou GR, Wang L, Liang L, Tian DM, Cao XL, Qin HY, Wang CM, Zhang P, Han H. (2013) "Inhibition of Tumor Angiogenesis and Tumor Growth by the DSL Domain of Human Delta-Like 1 Targeted to Vascular Endothelial Cells."?Neoplasia. 15 :815-25

36.) Lachance PA, Hazen A, Sevick-Muraca EM. (2013) "Lymphatic Vascular Response to Acute Inflammation."?PLoS One. 8: e76078.

37.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice"?BMC Complement Altern Med. 13: 168.?

38.) Cheng HS, Sivachandran N, Lau A, Boudreau E, Zhao JL, Baltimore D, Delgado-Olguin P, Cybulsky MI, Fish JE. (2013) "MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways."?EMBO Mol Med. 5: 1017-34.?

39.) Zhang W, Ni C, Sheng J, Hua Y, Ma J, Wang L, Zhao Y, Xing Y. (2013) 'TLQP-21 Protects Human Umbilical Vein Endothelial Cells against High-Glucose-Induced Apoptosis by Increasing G6PD Expression.'

40.) Zhang S, Liu L, Wang R, Tuo H, Guo Y, Yi L, Wang D, Wang J. (2013) 'MicroRNA-217 Promotes Angiogenesis of Human Cytomegalovirus-Infected Endothelial Cells through Downregulation of SIRT1 and FOXO3A.'

41.) Wu M, Zhao D, Zhong W, Yan H, Wang X, Liang Z, Li Z. (2013) 'High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.'

42.) Shi L, Zhang S, Wu H, Zhang L, Dai X, Hu J, Xue J, Liu T, Liang Y, Wu G. (2013) 'MiR-200c Increases the Radiosensitivity of Non-Small-Cell Lung Cancer Cell Line A549 by Targeting VEGF-VEGFR2 Pathway.'

43.) Muniz-Feliciano L, Van GJ, Portillo JAC, Liew L, Liu B, Carlin CR, Carruthers VB, Matthews S, Subauste CS. (2013) 'Toxoplasma gondii-Induced Activation of EGFR Prevents Autophagy Protein-Mediated Killing of the Parasite.'

44.) Kong X, Zhang Y, Liu C, Guo W, Li X, Su X, Wan H, Sun Y, Lin, N. (2013) 'Anti-Angiogenic Effect of Triptolide in Rheumatoid Arthritis by Targeting Angiogenic Cascade.'

45.) Kong X, Zhang Y, Liu C, Guo W, Li X, Su X, Wan H, Sun Y, Lin, N. (2013) "Anti-Angiogenic Effect of Triptolide in Rheumatoid Arthritis by Targeting Angiogenic Cascade."PloS one. 8: e77513.

46.) Muniz-Feliciano L, Van GJ, Portillo JAC, Liew L, Liu B, Carlin CR, Carruthers VB, Matthews S, Subauste CS. (2013) "Toxoplasma gondii-Induced Activation of EGFR Prevents Autophagy Protein-Mediated Killing of the Parasite."?PLoSPathog. 9: e1003809.

47.) Shi L, Zhang S, Wu H, Zhang L, Dai X, Hu J, Xue J, Liu T, Liang Y, Wu G. (2013) "MiR-200c Increases the Radiosensitivity of Non-Small-Cell Lung Cancer Cell Line A549 by Targeting VEGF-VEGFR2 Pathway."?PloS one. 8: e78344.

48.) Wu M, Zhao D, Zhong W, Yan H, Wang X, Liang Z, Li Z. (2013) "High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes."?Sci rep. 3.

49.) Zhang S, Liu L, Wang R, Tuo H, Guo Y, Yi L, Wang D, Wang J. (2013) "MicroRNA-217 Promotes Angiogenesis of Human Cytomegalovirus-Infected Endothelial Cells through Downregulation of SIRT1 and FOXO3A."?PloS one. 8: e83620.

50.) Zhang W, Ni C, Sheng J, Hua Y, Ma J, Wang L, Zhao Y, Xing Y. (2013) "TLQP-21 Protects Human Umbilical Vein Endothelial Cells against High-Glucose-Induced Apoptosis by Increasing G6PD Expression."?PloS one. 8: e79760.

51.) Claessens A, Adams Y, Ghumra A, Lindergard G, Buchan CC, Andisi C, Bull PC, Mok S, Gupta AP, Wang CW, Turner L, Arman M, Raza A, Bozdech Z, Rowe JA. (2012) "A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells."?Proc Natl Acad Sci USA. 109: E1772-81.?

52.) Feng S, Cen J, Huang Y, Shen H, Yao L, Wang Y, Chen Z. (2011) "Matrix metalloproteinase-2 and -9 secreted by leukemic cells increase the permeability of blood-brain barrier by disrupting tight junction proteins."?PLoS One. 6: e20599.

53.) Sun J, Wang S, Zhao D, Hun FH, Weng L, Liu H. (2011) "Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles in human cardiac microvascular endothelial cells: cytotoxicity, permeability, and inflammation of metal oxide nanoparticles."?Cell Biol Toxicol. 27: 333-42.?

54.) Dunn J, Gutbrod S, Webb A, Pak A, Jandu SK, Bhunia A, Berkowitz DE, Santhanam L. (2011) S-nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase.?Mol Cell Biochem. 355: 83-9.

55.) Ko YC, Chien HF, Jiang-Shieh YF, Chang CY, Pai MH, Huang JP, Chen HM, Wu CH. (2009) "Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions."?J Anat. 214: 183-95.?

56.) Solito R, Corti F, Fossati S, Mezhericher E, Donnini S, Ghiso J, Giachetti A, Rostagno A, Ziche M. (2009) "Dutch and Arctic mutant peptides of beta amyloid(1-40) differentially affect the FGF-2 pathway in brain endothelium."?Exp Cell Res. 315: 385-95.

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60.) Sharp CD, Hines I, Houghton J, Warren A, Jackson TH 4th, Jawahar A, Nanda A, Elrod JW, Long A, Chi A, Minagar A, Alexander JS. (2003) "Glutamate causes a loss in human cerebral endothelial barrier integrity through activation of NMDA receptor."?Am J Physiol Heart Circ Physiol. 285: H2592-8.

Question:

I am just wondering this medium can be used for the culture of murine endothelial cells? Thank you very much!

Answers:

  • Posted by Manoj Sharma on Tuesday, January 12, 2016

    We never tested the medium for murine cells


Question:

could you tell us the composition of media 1001--we just need to compare to another company's product we are using

Answers:

  • Posted by Manoj Sharma on Thursday, January 7, 2016

    Hello Grace,

    The complete composition of our medium is proprietary so we cannot share with customers. However, if you would like to know the concentration of some specific components, we will be more than happy to help you.thanks for your interest

    SCIENCELL


Question:

Hi,is it suitable for culture of rat endothelial cells?

Answers:

  • Posted by Manoj Sharma on Monday, August 17, 2015

    Yes.

特殊培养基
特殊培养基
货号 产品名称 规格 价格 指令
1001 内皮细胞培养基 ECM 500ml $139 +
1001-GF 内皮细胞培养基-不含葡萄糖ECM-GF 500ml $139 +
1001-NG 内皮细胞培养基-不含谷胺酰氨 ECM-NG 500ml $139 +
1001-prf 无酚红-内皮细胞培养基 ECM-prf 500ml $146 +
1021 内皮细胞培养基-大鼠 500 Ml $139 +
1101 平滑肌细胞培养基 SMCM 500ml $126 +
1111 平滑肌细胞培养基 SMCM-sf 500ml $151 +
1201 周边细胞培养基 PM 500ml $132 +
1231 周细胞培养基-小鼠 PM-m 500ml $132 +
1401 脑膜细胞培养基 MenCM 500ml $132 +
1511 神经前体细胞分化培养基 NPCDM 500ml $154 +
1521 神经细胞培养基 NM 500ml $139 +
1531 神经前体细胞的分化培养基 NPCDM 500ml $135 +
1601 少突胶质前体细胞培养基 OPCM 500ml $204 +
1611 球状少突细胞培养基 OsM 500ml $204 +
1621 少突胶质细胞培养基 OM 500ml $139 +
1631 少突胶质前体细胞分化培养基 OPCDM 500ml $139 +
1701 雪旺细胞培养基 SCM 500ml $139 +
1801 星形胶质细胞培养基 AM 500ml $127 +
1811 星形胶质细胞条件培养基 ACM 100ml $116 +
1811-sf 星形胶质细胞培养基-无血清 ACM-sf 100 ml $116 +
1831 动物星形胶质细胞培养基 AM-a 500ml $135 +
1901 小胶质细胞培养基 MM 500ml $139 +
1921 巨噬细胞培养基 MaM 500ml $139 +
2101 角质细胞培养基 KM 500ml $119 +
2111 角质细胞培养基(确定成分) KM-d 500ml $135 +
2121 角质细胞培养基-无动物成分 500ml $137 +
2201 黑色素细胞培养基 MelM 500ml $130 +
2211 黑色素细胞培养基-2 MelM-2 500ml $130 +
2301 成纤维细胞培养基 FM 500ml $127 +
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