The cornea is a unique tissue due to its transparency and avascularity. The cornea consists of three distinct cell layers: the outer epithelium, the central stroma, and the inner endothelium. The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals to activate the corneal defense system. Corneal epithelial cells also express a high level of aldehyde dehydrogenase to protect them against UV- and 4-hydroxynonenal-induced cellular damage. The renewal of corneal epithelium is regulated by a host of cytokines, including epidermal growth factor, which activates their cognate receptors in the deeper layers of the epithelium. Cultured corneal epithelial cells can be an alternative to animal testing in toxicology studies. They can also be used to understand the highly integrated balance between corneal epithelial proliferation, differentiation, and cell death. HCEpiC from ScienCell Research Laboratories are isolated from human cornea. HCEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCEpiC are characterized by their cobble stone morphology in serum-free culture and immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Corneal Epithelial Cell Medium (CEpiCM, Cat. #6511) for culturing HCEpiC in vitro.