Uric acid is the end product of the metabolic breakdown of purine nucleotides. Uric acid is dissolved in the blood and passes through the kidneys into the urine. When more uric acid is produced than the kidneys can eliminate, the elevated level is known as hyperuricemia, which is associated with insulin resistance, cardiovascular disease, and gout. Increased serum uric acid can be a marker of renal disease. This colorimetric assay is based on uricase-catalyzed oxidation of uric acid, in which the formed hydrogen peroxide is catalyzed by peroxidase and reacts with 4-aminoantipyrine to form the product dye. The color intensity of the reaction product at 540nm is directly proportional to uric acid concentration in the sample.

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