ScienCell's GeneQuery™ Human Pulmonary Fibrosis qPCR Array Kit (GQH-PFS) is designed to facilitate gene expression profiling of 88 key genes involved in pulmonary fibrosis. Pulmonary fibrosis is caused by excessive extracellular matrix (ECM) deposition during wound healing and tissue remodeling. The scarring of lung leads to thickened, stiff tissues and respiratory problems over time. Brief examples of how included genes may be grouped according to their functions are shown below:
- Pulmonary surfactant proteins: SFTPA2, SFTPAC
- Inflammatory chemokines: CCL2, CCL3, CCL11, CCR2, CCR3, CXCL8
- Inflammatory cytokines: PDGFA, CSF2, IL5, IL6, IL13
- ECM remodeling: LOX, MMP1, MMP2, MMP8, PLAT, PLAU, PLG, SERPINA1, SERPINE2, TIMP1, TIMP2, TIMP3
- Growth factors: EGF, IGF1, HGF, FGF1, FGF2, AGT, EDN1, CTGF
- TGFB signaling pathway: SKIL, SPP1, TGFB1, TGFB2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Pulmonary Fibrosis qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ACTA2 |
CCL3 |
CXCL8 |
FAM13A |
HPS3 |
IL13 |
MMP1 |
NFE2L2 |
PLAU |
SFTPD |
TGFA |
β-actin |
|
B |
AGT |
CCL5 |
CYSLTR2 |
FGF1 |
HPS4 |
IL13RA2 |
MMP13 |
NFKB1 |
PLG |
SKIL |
TGFB1 |
GAPDH |
|
C |
ATP11A |
CCR2 |
DSP |
FGF2 |
HPS5 |
IL1B |
MMP2 |
NFKB2 |
PTX3 |
SMAD3 |
TGFB2 |
LDHA |
|
D |
BMP7 |
CCR3 |
EDN1 |
FGF7 |
HPS6 |
IL5 |
MMP3 |
OBFC1 |
SERPINA1 |
SMAD7 |
TIMP1 |
NONO |
|
E |
CALCA |
CEBPB |
EGF |
GREM1 |
IFNG |
IL6 |
MMP8 |
PARN |
SERPINE1 |
SNAI1 |
TIMP2 |
PPIH |
|
F |
CCL11 |
CSF2 |
ELMOD2 |
HGF |
IGF1 |
LOX |
MMP9 |
PDGFA |
SERPINH1 |
SPP1 |
TIMP3 |
GDC |
|
G |
CCL18 |
CSF3 |
ELN |
HMOX1 |
IL10 |
LPAR1 |
MT2A |
PDGFB |
SFTPA2 |
TERC |
TIMP4 |
PPC |
|
H |
CCL2 |
CTGF |
FAM111B |
HPS1 |
IL12B |
MECP2 |
MUC5B |
PLAT |
SFTPC |
TERT |
TNF |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK034 |
| 产地 | 美国 |
| 缩写 | GQH-PFS |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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