ScienCell's GeneQuery™ Human Focal Adhesion Complexes qPCR Array Kit (GQH-FAC) profiles 88 key genes involved in the regulation and formation of focal adhesions. Focal adhesions are large macromolecular complexes by which the cell interacts with its extracellular matrix (ECM) environment. They are involved in such diverse processes as interpreting mechanical force, regulating cell adhesion and motility, and contributing to intracellular signaling transduction. Integrins and focal adhesion kinase (PTK2) are the main regulatory elements of focal adhesions. Brief examples of how included genes may be categorized are shown below:
- FAK signaling: CCND1, FYN, PTK2, RHOA, SRC
- Cytoskeletal elements: ACTB, ACTN1, ACTN3, FLNA, VCL
- ECM components: COL1A2, COL2A1, COL4A2, FN1, LAMC3
- ECM binding elements: ITGA1, ITGA5, ITGAM, ITGB1, ITGB3
- Signaling transduction genes: ARHGAP5, ELK1, HGF, JUN, KDR
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Focal Adhesion Complexes qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACTB |
BRAF |
COL4A2 |
FYN |
ITGA3 |
ITGAL |
ITGB5 |
MAP2K1 |
PAK2 |
PXN |
SHC1 |
β-actin |
|
B |
ACTN1 |
CAV1 |
CRK |
GRB2 |
ITGA4 |
ITGAM |
ITGB6 |
MAPK1 |
PAK4 |
RAC1 |
SHC3 |
GAPDH |
|
C |
ACTN3 |
CAV2 |
DOCK1 |
HGF |
ITGA5 |
ITGAV |
ITGB7 |
MAPK10 |
PDGFRA |
RAF1 |
SOS1 |
LDHA |
|
D |
AKT1 |
CAV3 |
EGFR |
HRAS |
ITGA6 |
ITGAX |
JUN |
MAPK8 |
PDGFRB |
RAP1A |
SRC |
NONO |
|
E |
AKT2 |
CCND1 |
ELK1 |
ILK |
ITGA7 |
ITGB1 |
KDR |
MAPK9 |
PDPK1 |
RAPGEF1 |
TLN1 |
PPIH |
|
F |
AKT3 |
CDC42 |
FLNA |
ITGA1 |
ITGA8 |
ITGB2 |
LAMA1 |
MET |
PIK3R1 |
RHOA |
VAV3 |
GDC |
|
G |
ARHGAP5 |
COL1A2 |
FLT1 |
ITGA2 |
ITGAD |
ITGB3 |
LAMB1 |
MYLK2 |
PIK3R2 |
ROCK1 |
VCL |
PPC |
|
H |
BCAR1 |
COL2A1 |
FN1 |
ITGA2B |
ITGAE |
ITGB4 |
LAMC3 |
PAK1 |
PTK2 |
ROCK2 |
VTN |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK106 |
| 产地 | 美国 |
| 缩写 | GQH-FAC |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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