ScienCell's GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:
- T cell receptor complex components: CD3D, CD3E, CD3G
- Co-receptors: CD4, CD8A, CD8B
- Co-inhibitors: PDCD1, CTLA4
- Co-stimulators: ICOS, CD28
- Kinases: FYN, ZAP70
- MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
- PI3K/AKT pathway: PI3Ks, AKT1, AKT2
- IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AKT1 |
CD274 |
CD8A |
DYRK1A |
HRAS |
MALT1 |
NCK1 |
NFKBIE |
PDPK1 |
PPP3CB |
RELA |
β-actin |
|
B |
AKT2 |
CD28 |
CD8B |
DYRK2 |
ICOS |
MAP2K1 |
NCK2 |
NRAS |
PIK3CA |
PPP3R1 |
SOS1 |
GAPDH |
|
C |
BCL10 |
CD3D |
CDC42 |
FOS |
IKBKB |
MAP2K7 |
NFATC1 |
NRON |
PIK3CB |
PRKCA |
SOS2 |
LDHA |
|
D |
CALM1 |
CD3E |
CDK4 |
FYN |
IKBKG |
MAP3K7 |
NFATC2 |
PAK1 |
PIK3R1 |
PRKCQ |
TEC |
NONO |
|
E |
CARD11 |
CD3G |
CHUK |
GRAP2 |
ITK |
MAP3K8 |
NFATC3 |
PAK2 |
PIK3R2 |
PTCRA |
TNF |
PPIH |
|
F |
CBL |
CD4 |
CTLA4 |
GRB2 |
JUN |
MAPK1 |
NFKB1 |
PAK3 |
PIK3R3 |
PTPN6 |
VAV1 |
GDC |
|
G |
CBLB |
CD80 |
DAG1 |
GSK3A |
KRAS |
MAPK3 |
NFKBIA |
PAK4 |
PLCG1 |
RAF1 |
VAV2 |
PPC |
|
H |
CBLC |
CD86 |
DLG1 |
GSK3B |
LCP2 |
MAPK9 |
NFKBIB |
PDCD1 |
PPP3CA |
RASGRP1 |
ZAP70 |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK021 |
| 产地 | 美国 |
| 缩写 | GQH-TRS |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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