ScienCell's GeneQuery™ human pluripotent stem cell qPCR array kit (GQH-hPSC) is designed to facilitate gene expression profiling of key genes involved in maintaining pluripotency and self-renewal of hPSC, and genes regulated during differentiation of hPSC. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Pluripotent Stem Cell qPCR Array Plate Layout* (8 controls in Bold and Italic)
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|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ACTC1 |
CDX2 |
EN2 |
FOXA2 |
GFAP |
ISL1 |
MIXL1 |
NOG |
PECAM1 |
SOX1 |
TBX6 |
β-actin |
|
B |
AFP |
CER1 |
ENO2 |
FOXD3 |
GJA1 |
KLF4 |
MYF5 |
NPM1 |
POU5F1 |
SOX2 |
TCF2 |
GAPDH |
|
C |
ALB |
COL1A1 |
EOMES |
FOXJ3 |
GSC |
LEFTYA |
MYOD1 |
NR5A2 |
PTF1A |
SOX7 |
TCF3 |
LDHA |
|
D |
ALDH1A1 |
COL2A1 |
ESG1 |
GATA2 |
HAND1 |
LEFTYB |
NANOG |
OLIG2 |
REST |
SOX17 |
TDGF1 |
NONO |
|
E |
BRIX1 |
CXCR4 |
ESRRB |
GATA4 |
HDAC2 |
LIN28 |
NEUROD |
OTX2 |
REX1 |
T |
TERT |
PPIH |
|
F |
CD34 |
DES |
FGF4 |
GATA6 |
HLXB9 |
MEF2C |
NCAM1 |
PAX2 |
RUNX1 |
TAT |
TUBB3 |
GDC |
|
G |
CD90 |
DNMT3B |
FGF5 |
GBX2 |
HNF4A |
MEIS1 |
NES |
PAX6 |
RUNX2 |
TBX3 |
UTF1 |
PPC |
|
H |
CDH1 |
EMX2 |
FLT1 |
GDF3 |
IDH1 |
MESP1 |
NKX2.5 |
PDX-1 |
SALL4 |
TBX5 |
VIM |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK002 |
| 产地 | 美国 |
| 缩写 | GQH-hPSC |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK101 | 人类Synoviocyte细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK102 | 人类滋养层细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK103 | 人前脂肪细胞生物学qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK105 | 人类ECM降解qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK106 | 人类焦点粘附复合物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK107 | 人内皮素信号qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK111 | 人凝集素调节免疫应答qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK112 | 人类神经分化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK113 | 人类神经干细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK114 | 人类小胶质细胞极化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK115 | 人类骨髓谱系标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK116 | 人B细胞和T细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK117 | 人胸腺细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK118 | 人类先天淋巴细胞发育标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK119 | 人类神经祖细胞标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK121 | 人PD-1 / PD-L1检查点途径qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK123 | 人类生长因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK124 | 人类转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK125 | 人干细胞转录因子qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK126 | 人类细胞表面标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK127 | 人脑发育标记物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
