Hypoxia refers to any environment with reduced oxygen content compared to normal aerobic conditions.? The hypoxic response is induced when cells are oxygen deprived and prolonged deprivation often results in physiological compensation by the local cellular environment, such as increased angiogenesis to the hypoxic region.? ScienCell's GeneQuery? Human Hypoxia Response qPCR Array Kit (GQH-HPX) is designed to facilitate gene expression profiling of 88 key genes involved in the cellular response to low-oxygen conditions.? Brief examples of how included genes may be grouped according to response type are shown below:
- Cellular metabolism: ENO1, HK1, PFKFB3, PGK1, SLC2A1
- Inflammation: CXCL8, IL1A, CCL2, IL6, THBD
- Vasorelaxation: AHSP, PTGIS
- Cell death or proliferation: BNIP3L, NOS3, PIM1, EPO
- Angiogenesis: KDR, PDGFB, PLAU, TEK, FLT1
GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.?
GeneQuery? Human Endothelial Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
?
| ? |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AHSP |
BNIP3L |
EGLN1 |
EPO |
HIF3A |
JMJD6 |
OS9 |
PIK3CA |
PTGIR |
SAT2 |
TEK |
β-actin |
|
B |
ALDOA |
CA9 |
EGLN2 |
F2 |
HK1 |
KDR |
PDGFB |
PIM1 |
PTGIS |
SELP |
THBD |
GAPDH |
|
C |
ANXA2 |
CCL2 |
EGLN3 |
FLT1 |
HRH1 |
LDHA |
PDPK1 |
PLAU |
PTGS2 |
SENP1 |
TNF |
LDHA |
|
D |
APEX1 |
COPS5 |
EGR1 |
FOXO4 |
ICAM1 |
LGALS3 |
PECAM1 |
PPP3CB |
RACK1 |
SLC2A1 |
TP53 |
NONO |
|
E |
ARNT |
CREBBP |
EIF4E |
GSK3B |
IFNG |
LTC4S |
PER1 |
PPP3CC |
RPS6 |
SP1 |
TXN |
PPIH |
|
F |
ARNT2 |
CUL2 |
ENO1 |
GYS1 |
IL1A |
MAP3K1 |
PFKFB3 |
PRKCA |
RUVBL2 |
STAT3 |
USP20 |
GDC |
|
G |
ARNTL |
CXCL8 |
EP300 |
HIF1A |
IL4 |
NFKB1 |
PFKFB4 |
PSMC6 |
RWDD3 |
TCEB1 |
VCAM1 |
PPC |
|
H |
BNIP3 |
EDN1 |
EPAS1 |
HIF1AN |
IL6 |
NOS3 |
PGK1 |
PTGFR |
SAT1 |
TCEB2 |
VHL |
NTC |
?
?* gene selection may be updated based on new research and development
| 货号 | GK035 |
| 产地 | 美国 |
| 缩写 | GQH-HPX |
| 规格 | 96tests |
| 用途 | 科研 |
| 储存 | 4度 |
| 运输 | 干冰 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 | |
| GK091 | 人肝星状细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK092 | 人胰腺星状细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK093 | Human Renal Mesangial Cell Biology qPCR Array Kit | 96Tests | $286 | + | |
| GK094 | Human Mesenchymal Stem Cell Differentiation qPCR Array | 96tests | $286 | + | |
| GK094 | Human Mesenchymal Stem Cell Differentiation qPCR Array | 96Tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK095 | 人间充质至上皮细胞转化qPCR检测试剂盒 | 96tests | $286 | + | |
| GK096 | 人类雪旺细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK097 | 人类平滑肌细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK103 | 人前脂肪细胞生物学qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK104 | 人类细胞骨架重塑qPCR检测试剂盒 | 96Tests | $286 | + | |
| GK105 | 人类ECM降解qPCR检测试剂盒 | 96tests | $286 | + | |
| GK112 | 人类神经分化标记qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + | |
| GK127 | 人脑发育标记物qPCR检测试剂盒 | One 96-well plate, one 20ul reaction per well | $286 | + |
