ScienCell's GeneQuery™ Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:
Note : all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Brain Development Markers qPCR Array Plate Layout*
(8 controls in Bold and Italic)
Note : all gene names follow their official symbols by HGNC
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
TNF |
NOS2 |
IL1B |
ACTB |
TNF |
NOS2 |
IL1B |
ACTB |
TNF |
NOS2 |
IL1B |
ACTB |
B |
IL10 |
ARG1 |
MRC1 |
GAPDH |
IL10 |
ARG1 |
MRC1 |
GAPDH |
IL10 |
ARG1 |
MRC1 |
GAPDH |
C |
ACTA2 |
PDGFRB |
CSPG4 |
LDHA |
ACTA2 |
PDGFRB |
CSPG4 |
LDHA |
ACTA2 |
PDGFRB |
CSPG4 |
LDHA |
D |
GFAP |
S100B |
GLUL |
NONO |
GFAP |
S100B |
GLUL |
NONO |
GFAP |
S100B |
GLUL |
NONO |
E |
NEUROD1 |
DCX |
TUBB3 |
PPIH |
NEUROD1 |
DCX |
TUBB3 |
PPIH |
NEUROD1 |
DCX |
TUBB3 |
PPIH |
F |
MAP2 |
RBFOX3 |
SYP |
GDC |
MAP2 |
RBFOX3 |
SYP |
GDC |
MAP2 |
RBFOX3 |
SYP |
GDC |
G |
CHAT |
TH |
MAPT |
PPC |
CHAT |
TH |
MAPT |
PPC |
CHAT |
TH |
MAPT |
PPC |
H |
VCAM1 |
OCLN |
ICAM1 |
NTC |
VCAM1 |
OCLN |
ICAM1 |
NTC |
VCAM1 |
OCLN |
ICAM1 |
NTC |
* gene selection may be updated based on new research and development
货号 | GK127 |
产地 | 美国 |
缩写 | GQH-BDM |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |