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人脑发育标记物qPCR检测试剂盒

英文名:Human Brain Development Markers qPCR Array Kit

货号:GK127

价格:$250

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ScienCell's GeneQuery™ Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:

  • M1 microglia phenotype markers: TNF, NOS2, IL1B
  • M2 microglia phenotype markers: IL10, ARG1, MRC1
  • Pericyte markers: ACTA2, PDGFRB, CSPG4
  • Evaluation of neural differentiation
    • Astrocyte markers:   GFAP, S100B, GLUL
    • Immature neuron markers:   NEUROD1, DCX, TUBB3
    • Mature neuron markers:   MAP2, RBFOX3, SYP
  • Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
  • Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1

Note all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

 

GeneQuery™ Human Brain Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note : all gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

TNF

NOS2

IL1B

ACTB

TNF

NOS2

IL1B

ACTB

TNF

NOS2

IL1B

ACTB

B

IL10

ARG1

MRC1

GAPDH

IL10

ARG1

MRC1

GAPDH

IL10

ARG1

MRC1

GAPDH

C

ACTA2

PDGFRB

CSPG4

LDHA

ACTA2

PDGFRB

CSPG4

LDHA

ACTA2

PDGFRB

CSPG4

LDHA

D

GFAP

S100B

GLUL

NONO

GFAP

S100B

GLUL

NONO

GFAP

S100B

GLUL

NONO

E

NEUROD1

DCX

TUBB3

PPIH

NEUROD1

DCX

TUBB3

PPIH

NEUROD1

DCX

TUBB3

PPIH

F

MAP2

RBFOX3

SYP

GDC

MAP2

RBFOX3

SYP

GDC

MAP2

RBFOX3

SYP

GDC

G

CHAT

TH

MAPT

PPC

CHAT

TH

MAPT

PPC

CHAT

TH

MAPT

PPC

H

VCAM1

OCLN

ICAM1

NTC

VCAM1

OCLN

ICAM1

NTC

VCAM1

OCLN

ICAM1

NTC

 * gene selection may be updated based on new research and development

货号GK127
产地美国
缩写GQH-BDM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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