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人干细胞转录因子qPCR检测试剂盒

英文名:Human Stem Cell Transcription Factors qPCR Array Kit

货号:GK125

价格:$250

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ScienCell's GeneQuery™ Human Stem Cell Transcription Factors qPCR Array Kit (GQH-SCT) surveys a panel of 88 transcription factors related to stem cell maintenance and differentiation. Transcription factors are proteins that can regulate target gene transcription level by binding to specific regions of the genome known as enhancers or silencers. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Pluripotency transcription factors: NANOG, POU5F1, SOX2
  • Embryonic development: EOMES, FOXC2/D3/O1, HOXA9/A10, KLF2/5, LIN28B, MAX, PITX2, SMAD1, TBX5, ZIC1
  • Germ layer formation and differentiation: FOXA2, GATA6, HAND1, ISL1, KLF4, SMAD2, SOX9
  • Organ morphogenesis: EZH2, HOXA11/B3/B5/C9, MSX2, MYC, PAX5/8, VDR
  • Angiogenesis: CDX2, JUN, NR2F2, RUNX1, WT1
  • Hematopoiesis and osteogenesis: EGR3, ESR1, GATA1/2/3, GLI2, NFATC1, PAX9, RB1, SOX6, SP1, STAT1
  • Neural stem cell maintenance and differentiation: ATF2, CREB1, FOSB, FOXO3, HES1, HOXD10, MCM2/7, MEF2C, NEUROD1/G1, OLIG1/2, PAX3/6, POU4F1, PPARG, SMAD3/4, SNAI1, STAT3

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

 

GeneQuery™ Human Stem Cell Transcription Factors qPCR Array Plate Layout *

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ATF2

FOSB

FOXP3

HES1

ISL1

MAX

NEUROG1

PAX5

PPARG

SMAD4

STAT3

ACTB

B

CDX2

FOXA1

GATA1

HOXA10

JUN

MCM2

NFATC1

PAX6

PRDM16

SMARCA4

TBX5

GAPDH

C

CREB1

FOXA2

GATA2

HOXA11

KLF2

MCM7

NKX2-2

PAX8

PROX1

SNAI1

TCF3

LDHA

D

DNMT3B

FOXC2

GATA3

HOXA9

KLF4

MEF2C

NOTCH2

PAX9

RB1

SOX2

TDGF1

NONO

E

EGR3

FOXD3

GATA6

HOXB3

KLF5

MSX2

NR2F2

PITX2

RUNX1

SOX6

TLX3

PPIH

F

EOMES

FOXO1

GLI1

HOXB5

LIN28B

MYC

OLIG1

PITX3

SMAD1

SOX9

VDR

GDC

G

ESR1

FOXO3

GLI2

HOXC9

LMX1B

NANOG

OLIG2

POU4F1

SMAD2

SP1

WT1

PPC

H

EZH2

FOXP1

HAND1

HOXD10

MAF

NEUROD1

PAX3

POU5F1

SMAD3

STAT1

ZIC1

NTC

 

* gene selection may be updated based on new research and development

货号GK125
产地美国
缩写GQH-SCT
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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