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人类转录因子qPCR检测试剂盒

英文名:Human Transcription Factors qPCR Array Kit

货号:GK124

价格:$250

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ScienCell's GeneQuery™ Human Transcription Factors qPCR Array Kit (GQH-TRF) surveys a panel of 88 transcription factors. Transcription factors are proteins that can regulate target gene transcription level by binding to specific regions of genome known as enhancers or silencers. Misregulation of transcription factors can lead to aberrations in signaling transduction and a variety of disorders including autoimmunity, neurological disorders, diabetes, cardiovascular disease and cancer. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Stem cell-related: NANOG, POU5F1, SOX2, EGR1, FOXD3, FOXO1, KLF4, MEF2B, PAX6, SOX18
  • Cancer stem cell-related: NANOG, POU5F1, SOX2, AR, FOXO3, MYC, NKX3-1, SNAI1, SOX9, TFAP2A, TP53, TWIST1
  • Oxidative stress-related: ARNT, ETS1/2, IRF1, JUN, NFAT5, NFKB1, NFYA, SMAD1, SRF, STAT3
  • Neuroprotective function-related: CREB1, ESR1, FOXA1, GATA1/2/3, NFE2L2, NR4A2, PPARA, SP1, TBX3
  • Growth factor-mediated signaling pathways: CREBBP, ELK1, FOS, HIF1A, ID1, NFAT5, NFATs, STAT1/3/5A
  • Wnt signaling pathway: CEBPA, CEBPB, GBX2, HNF4A, LEF1, MITF, OLIG1, PITX2, SMAD4, SOX2
  • MAPK signaling pathway: ATF2/4, ELK1, FOX, JUN, MAX, MEF2A/C, NFKB1, SP1, STAT1/3
  • B/T-cell receptor signaling pathways: E2F1, ELK1, FOS, GATA3, JUN, MYB, NFATs, RUNX1, STAT6
  • Oncogenes & tumor suppressors: ABL1, BRCA1, CDKN2A, JUNB, MYCN, NF1, RARA, RB1, TGFB1, TNF

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

 

GeneQuery™ Human Transcription Factors qPCR Array Plate Layout *

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABL1

CEBPB

ETS1

GATA2

IRF1

MEF2C

NFATC1

NR4A2

REL

SOX18

STAT6

ACTB

B

AR

CREB1

ETS2

GATA3

JUN

MITF

NFATC2

OLIG1

RELA

SOX2

TBX3

GAPDH

C

ARNT

CREBBP

FOS

GBX2

JUNB

MYB

NFATC3

PAX6

RUNX1

SOX9

TFAP2A

LDHA

D

ATF2

CTNNB1

FOXA1

GLI1

KLF4

MYC

NFATC4

PITX2

SMAD1

SP1

TGFB1

NONO

E

ATF4

E2F1

FOXD3

HIF1A

LEF1

MYCN

NFE2L2

POU5F1

SMAD4

SRF

TNF

PPIH

F

BRCA1

EGR1

FOXO1

HNF4A

MAX

NANOG

NFKB1

PPARA

SMAD5

STAT1

TP53

GDC

G

CDKN2A

ELK1

FOXO3

HSF1

MEF2A

NF1

NFYA

RARA

SMAD9

STAT3

TWIST1

PPC

H

CEBPA

ESR1

GATA1

ID1

MEF2B

NFAT5

NKX3-1

RB1

SNAI1

STAT5A

WT1

NTC

 

* gene selection may be updated based on new research and development

货号GK124
产地美国
缩写GQH-TRF
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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