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人类神经祖细胞标记qPCR检测试剂盒

英文名:Human Neural Progenitor Markers qPCR Array Kit

货号:GK119

价格:$250

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ScienCell's GeneQuery™ Human Neural Progenitor Markers qPCR Array Kit (GQH-NPM) is designed to facilitate gene expression profiling of 40 marker genes commonly used to assess neural progenitor cell (NPC) maintenance, proliferation, and differentiation. NPCs are generated by asymmetric mitosis of neural stem cells (NSCs), in which one NSC becomes one NSC and one NPC, or by terminal asymmetric division, in which one NSC becomes two NPCs. The multipotent NPCs have limited differentiation capacity and can differentiate into 3 cell types: neurons, astrocytes and oligodendrocytes.

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Neural Progenitor Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AP2A1

FZD4

MSX1

NTN4

SEMA7A

β-actin

AP2A1

FZD4

MSX1

NTN4

SEMA7A

β-actin

B

ASCL1

GAP43

NCAM1

OTX2

SMARCA4

GAPDH

ASCL1

GAP43

NCAM1

OTX2

SMARCA4

GAPDH

C

ATP1A1

GFAP

NES

PAX3

SOX1

LDHA

ATP1A1

GFAP

NES

PAX3

SOX1

LDHA

D

CD4

HMMR

NEUROD1

PAX6

SOX2

NONO

CD4

HMMR

NEUROD1

PAX6

SOX2

NONO

E

DCX

ID2

NOTCH1

ROR2

TNFRSF19

PPIH

DCX

ID2

NOTCH1

ROR2

TNFRSF19

PPIH

F

EOMES

INA

NOTCH2

S100B

TUBB2A

GDC

EOMES

INA

NOTCH2

S100B

TUBB2A

GDC

G

FABP7

JAG1

NOTCH3

SEMA3C

TUBB3

PPC

FABP7

JAG1

NOTCH3

SEMA3C

TUBB3

PPC

H

FUT4

MSI1

NTN1

SEMA6B

VIM

NTC

FUT4

MSI1

NTN1

SEMA6B

VIM

NTC

 

* gene selection may be updated based on new research and development

货号GK119
产地美国
缩写GQH-NPM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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