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人B细胞和T细胞发育标记qPCR检测试剂盒

英文名:Human B and T Cells Development Markers qPCR Array Kit

货号:GK116

价格:$250

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ScienCell's GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit (GQH-BTD) is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells. Brief examples of how included genes may be grouped according to their functions are shown below:

  • CLP markers: MME, CD34, IL7R, FLT3
  • ProB-PreB-immature B cell markers: MME, MS4A1, SPN, PTPRC, IL7R, CD79A/B, CD40, CD19
  • B1 cell markers: CD19, MS4A1, CD27, SPN, CD40LG
  • Regulatory B cell markers: CD1D, CD5, CD19, CR2, CD24
  • Marginal Zone B cell markers: CD1C, CD19, MS4A1, CR2, CD24
  • Follicular B cell markers: CD19, MS4A1, CR2, CD22, FCER2
  • Memory B cell markers: CD27, CD80, CD84, CD86
  • Plasmablast-plasma cell markers: CD19, CD27, CD38, IL6R, SDC1, SLAMF7
  • Double-negative and double-positive stage T cell markers: CD1A, CD3D/E/G, CD4, CD5, CD7, CD8A/B, CD38, SELE/L/P, CD160, CD200                       

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human B and T Cells Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

CD160

CD27

CD40LG

CD8A

MS4A1

ACTB

CD160

CD27

CD40LG

CD8A

MS4A1

ACTB

B

CD19

CD34

CD5

CD8B

PTPRC

GAPDH

CD19

CD34

CD5

CD8B

PTPRC

GAPDH

C

CD1A

CD38

CD7

CR2

SDC1

LDHA

CD1A

CD38

CD7

CR2

SDC1

LDHA

D

CD1C

CD3D

CD79A

FCER2

SELE

NONO

CD1C

CD3D

CD79A

FCER2

SELE

NONO

E

CD1D

CD3E

CD79B

FLT3

SELL

PPIH

CD1D

CD3E

CD79B

FLT3

SELL

PPIH

F

CD200

CD3G

CD80

IL6R

SELP

GDC

CD200

CD3G

CD80

IL6R

SELP

GDC

G

CD22

CD4

CD84

IL7R

SLAMF7

PPC

CD22

CD4

CD84

IL7R

SLAMF7

PPC

H

CD24

CD40

CD86

MME

SPN

NTC

CD24

CD40

CD86

MME

SPN

NTC

 

* gene selection may be updated based on new research and development

货号GK116
产地美国
缩写GQH-BTD
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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