ScienCell's GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit (GQH-BTD) is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells. Brief examples of how included genes may be grouped according to their functions are shown below:
Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human B and T Cells Development Markers qPCR Array Plate Layout*
(8 controls in Bold and Italic)
Note: all gene names follow their official symbols by HGNC
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
CD160 |
CD27 |
CD40LG |
CD8A |
MS4A1 |
ACTB |
CD160 |
CD27 |
CD40LG |
CD8A |
MS4A1 |
ACTB |
B |
CD19 |
CD34 |
CD5 |
CD8B |
PTPRC |
GAPDH |
CD19 |
CD34 |
CD5 |
CD8B |
PTPRC |
GAPDH |
C |
CD1A |
CD38 |
CD7 |
CR2 |
SDC1 |
LDHA |
CD1A |
CD38 |
CD7 |
CR2 |
SDC1 |
LDHA |
D |
CD1C |
CD3D |
CD79A |
FCER2 |
SELE |
NONO |
CD1C |
CD3D |
CD79A |
FCER2 |
SELE |
NONO |
E |
CD1D |
CD3E |
CD79B |
FLT3 |
SELL |
PPIH |
CD1D |
CD3E |
CD79B |
FLT3 |
SELL |
PPIH |
F |
CD200 |
CD3G |
CD80 |
IL6R |
SELP |
GDC |
CD200 |
CD3G |
CD80 |
IL6R |
SELP |
GDC |
G |
CD22 |
CD4 |
CD84 |
IL7R |
SLAMF7 |
PPC |
CD22 |
CD4 |
CD84 |
IL7R |
SLAMF7 |
PPC |
H |
CD24 |
CD40 |
CD86 |
MME |
SPN |
NTC |
CD24 |
CD40 |
CD86 |
MME |
SPN |
NTC |
* gene selection may be updated based on new research and development
货号 | GK116 |
产地 | 美国 |
缩写 | GQH-BTD |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |