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人类骨髓谱系标记qPCR检测试剂盒

英文名:Human Myeloid Lineage Markers qPCR Array Kit

货号:GK115

价格:$250

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ScienCell's GeneQuery™ Human Myeloid Lineage Markers qPCR Array Kit (GQH-MLM) is designed to facilitate gene expression profiling of 40 marker genes involved in myelopoiesis. In the tightly regulated lineage of common myeloid progenitors (CMPs), the CMPs can first differentiate into granulocytes, monocytes, mast cells and macrophages. The granulocytes can further differentiate into mature eosinophils, basophils, and neutrophils. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • CMP markers: CD34, CD38, KIT, FLT3
  • Granulocyte-monocyte precursor markers: CD34, CD38, CD48, IL3RA, FLT3
  • Monocyte markers: ITGAM, CD14, PECAM1, SELL, CSF1R, CCR2, FCGR3A, FCGR3B, CXCR3
  • Macrophage markers: ITGAM, ITGAX, CD14, CD68, CD80, CD163
  • Polarized macrophage markers: CD68, CD86, TLR2, TLR4, CIITA, CD163, CD200R1, MRC1, CD81, VCAM1, CLEC7A, VEGFA
  • Mast cell markers: FCER2, KIT, ENPP3, FCER1A
  • Neutrophil markers: ANPEP, FUT4, CD33
  • Eosinophil markers: CD9, FCER2, FCGR2A, FCGR2B, FCGR2C
  • Basophil markers: CD69, IL3RA, FCER1A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Myeloid Lineage Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ANPEP

CD48

CLEC7A

FCGR2C

KIT

β-actin

ANPEP

CD48

CLEC7A

FCGR2C

KIT

β-actin

B

CCR2

CD68

CSF1R

FCGR3A

MRC1

GAPDH

CCR2

CD68

CSF1R

FCGR3A

MRC1

GAPDH

C

CD14

CD69

CXCR3

FCGR3B

PECAM1

LDHA

CD14

CD69

CXCR3

FCGR3B

PECAM1

LDHA

D

CD163

CD80

ENPP3

FLT3

SELL

NONO

CD163

CD80

ENPP3

FLT3

SELL

NONO

E

CD200R1

CD81

FCER1A

FUT4

TLR2

PPIH

CD200R1

CD81

FCER1A

FUT4

TLR2

PPIH

F

CD33

CD86

FCER2

IL3RA

TLR4

GDC

CD33

CD86

FCER2

IL3RA

TLR4

GDC

G

CD34

CD9

FCGR2A

ITGAM

VCAM1

PPC

CD34

CD9

FCGR2A

ITGAM

VCAM1

PPC

H

CD38

CIITA

FCGR2B

ITGAX

VEGFA

NTC

CD38

CIITA

FCGR2B

ITGAX

VEGFA

NTC

 

* gene selection may be updated based on new research and development

货号GK115
产地美国
缩写GQH-MLM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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