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人类小胶质细胞极化标记qPCR检测试剂盒

英文名:Human Microglial Polarization Markers qPCR Array Kit

货号:GK114

价格:$250

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ScienCell's GeneQuery™ Human Microglial Polarization Markers qPCR Array Kit (GQH-MPM) is designed to facilitate gene expression profiling of 40 marker genes involved in microglial polarization, which has important implications for regulating innate immunological functions. When microglia are stimulated, two potential phenotypes can be observed: pro-inflammatory M1 microglia and anti-inflammatory M2 microglia. The M1 phenotype acts to protect tissues against invading pathogens by releasing cytokines and revoking acute inflammation, while the M2 phenotype acts to restore tissue homeostasis and stimulate tissue repair. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • M1 phenotype marker: TNF, FCGR2A, FCGR2B, FCGR2C, FCGR3A, FCGR3B, CD86, IL1B, NOS2, IL6
  • M2 phenotype marker: MRC1, IL4, IL10, TGFB1, IL1R1, SOCS3, ARG1, IL13, SPHK1
  • Activation/resting markers: CD80, CLEC5A, HEXB, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, IRF8, ITGAM, MYB, PTGES, RUNX1, TLR3, TLR4
  • Additional microglia markers: ADGRE1, AIF1, CD40, CD68, EDA, ITGAM, ITGAX, PTPRC

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Microglial Polarization Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADGRE1

EDA

HLA-DQB1

IL6

PTPRC

β-actin

ADGRE1

EDA

HLA-DQB1

IL6

PTPRC

β-actin

B

AIF1

FCGR2A

HLA-DRA

IRF8

RUNX1

GAPDH

AIF1

FCGR2A

HLA-DRA

IRF8

RUNX1

GAPDH

C

ARG1

FCGR2B

HLA-DRB1

ITGAM

SOCS3

LDHA

ARG1

FCGR2B

HLA-DRB1

ITGAM

SOCS3

LDHA

D

CD40

FCGR2C

IL10

ITGAX

SPHK1

NONO

CD40

FCGR2C

IL10

ITGAX

SPHK1

NONO

E

CD68

FCGR3A

IL13

MRC1

TGFB1

PPIH

CD68

FCGR3A

IL13

MRC1

TGFB1

PPIH

F

CD80

FCGR3B

IL1B

MYB

TLR3

GDC

CD80

FCGR3B

IL1B

MYB

TLR3

GDC

G

CD86

HEXB

IL1R1

NOS2

TLR4

PPC

CD86

HEXB

IL1R1

NOS2

TLR4

PPC

H

CLEC5A

HLA-DQA1

IL4

PTGES

TNF

NTC

CLEC5A

HLA-DQA1

IL4

PTGES

TNF

NTC

 

* gene selection may be updated based on new research and development

货号GK114
产地美国
缩写GQH-MPM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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