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人类神经分化标记qPCR检测试剂盒

英文名:Human Neural Differentiation Markers qPCR Array Kit

货号:GK112

价格:$250

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ScienCell's GeneQuery™ Human Neural Differentiation Markers qPCR Array Kit (GQH-NDM) is designed to facilitate gene expression profiling of 40 marker genes involved in neural differentiation to specific neural lineages. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Immature neuron markers: DCX, NEUROD1, STMN1, TUBB3
  • Mature neuron markers: MAP2, NEFH, NEFM, RBFOX3
  • Astrocyte markers: GFAP, GLUL, S100B, SLC1A2, S100A6, NFIX, NFIC
  • Schwann cell markers: GAP43, MPZ, NCAM1, S100A1, NGFR, EGR2, POU3F1
  • Oligodendrocyte markers: CSPG4, MBP, MOG, OLIG1, OLIG2, PDGFRA, SOX10
  • Radial glial cell markers: CDH2, FABP7, GFAP, HES1, HES5, NES, PAX6, VIM
  • Neuroepithelial cell markers: CDH1, HES1, NES, NOTCH1, OCLN, SOX2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Neural Differentiation Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

CDH1

GLUL

NEFH

OCLN

S100A6

β-actin

CDH1

GLUL

NEFH

OCLN

S100A6

β-actin

B

CDH2

HES1

NEFM

OLIG1

S100B

GAPDH

CDH2

HES1

NEFM

OLIG1

S100B

GAPDH

C

CSPG4

HES5

NES

OLIG2

SLC1A2

LDHA

CSPG4

HES5

NES

OLIG2

SLC1A2

LDHA

D

DCX

MAP2

NEUROD1

PAX6

SOX10

NONO

DCX

MAP2

NEUROD1

PAX6

SOX10

NONO

E

EGR2

MBP

NFIC

PDGFRA

SOX2

PPIH

EGR2

MBP

NFIC

PDGFRA

SOX2

PPIH

F

FABP7

MOG

NFIX

POU3F1

STMN1

GDC

FABP7

MOG

NFIX

POU3F1

STMN1

GDC

G

GAP43

MPZ

NGFR

RBFOX3

TUBB3

PPC

GAP43

MPZ

NGFR

RBFOX3

TUBB3

PPC

H

GFAP

NCAM1

NOTCH1

S100A1

VIM

NTC

GFAP

NCAM1

NOTCH1

S100A1

VIM

NTC

 

* gene selection may be updated based on new research and development

货号GK112
产地美国
缩写GQH-NDM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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