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人凝集素调节免疫应答qPCR检测试剂盒

英文名:Human Lectin Regulation in Immune Response qPCR Array Kit

货号:GK111

价格:$250

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ScienCell's GeneQuery™ Human Lectin Regulation in Immune Response qPCR Array Kit (GQH-LRI) is designed to facilitate gene expression profiling of 40 selected lectin receptors that are involved in pathogen-specific immune responses. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • C-Type Lectin Receptors: ASGR1, CD69, CD207, CD302, CLEC3B, CLEC4A-M, CLEC5A, CLEC6A, CLEC7A, CLEC10A, CLEC16A, LY75, MRC1, MRC2
  • NK Cell lectin-like receptors: KLRB1, KLRC1, KLRC2, KLRD1, KLRK1
  • Other subgroups such as selectins, chondrolectins and collectins: ACAN, CD72,  CD93, CD248, MBL2, OLR1, PKD1, SELE, SELL, SELP, SFTPA2, SFTPD, THBD

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Lectin Regulation in Immune Response qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACAN

CD93

CLEC4E

KLRC1

OLR1

β-actin

ACAN

CD93

CLEC4E

KLRC1

OLR1

β-actin

B

ASGR1

CLEC10A

CLEC4M

KLRC2

PKD1

GAPDH

ASGR1

CLEC10A

CLEC4M

KLRC2

PKD1

GAPDH

C

CD207

CLEC12A

CLEC5A

KLRD1

SELE

LDHA

CD207

CLEC12A

CLEC5A

KLRD1

SELE

LDHA

D

CD209

CLEC16A

CLEC6A

KLRK1

SELL

NONO

CD209

CLEC16A

CLEC6A

KLRK1

SELL

NONO

E

CD248

CLEC3B

CLEC7A

LY75

SELP

PPIH

CD248

CLEC3B

CLEC7A

LY75

SELP

PPIH

F

CD302

CLEC4A

CLEC9A

MBL2

SFTPA2

GDC

CD302

CLEC4A

CLEC9A

MBL2

SFTPA2

GDC

G

CD69

CLEC4C

FCER2

MRC1

SFTPD

PPC

CD69

CLEC4C

FCER2

MRC1

SFTPD

PPC

H

CD72

CLEC4D

KLRB1

MRC2

THBD

NTC

CD72

CLEC4D

KLRB1

MRC2

THBD

NTC

 

* gene selection may be updated based on new research and development

货号GK111
产地美国
缩写GQH-LRI
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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