ScienCell's GeneQuery™ Human Lectin Regulation in Immune Response qPCR Array Kit (GQH-LRI) is designed to facilitate gene expression profiling of 40 selected lectin receptors that are involved in pathogen-specific immune responses. Brief examples of how included genes may be grouped according to their functions are shown below:
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Lectin Regulation in Immune Response qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
ACAN |
CD93 |
CLEC4E |
KLRC1 |
OLR1 |
β-actin |
ACAN |
CD93 |
CLEC4E |
KLRC1 |
OLR1 |
β-actin |
B |
ASGR1 |
CLEC10A |
CLEC4M |
KLRC2 |
PKD1 |
GAPDH |
ASGR1 |
CLEC10A |
CLEC4M |
KLRC2 |
PKD1 |
GAPDH |
C |
CD207 |
CLEC12A |
CLEC5A |
KLRD1 |
SELE |
LDHA |
CD207 |
CLEC12A |
CLEC5A |
KLRD1 |
SELE |
LDHA |
D |
CD209 |
CLEC16A |
CLEC6A |
KLRK1 |
SELL |
NONO |
CD209 |
CLEC16A |
CLEC6A |
KLRK1 |
SELL |
NONO |
E |
CD248 |
CLEC3B |
CLEC7A |
LY75 |
SELP |
PPIH |
CD248 |
CLEC3B |
CLEC7A |
LY75 |
SELP |
PPIH |
F |
CD302 |
CLEC4A |
CLEC9A |
MBL2 |
SFTPA2 |
GDC |
CD302 |
CLEC4A |
CLEC9A |
MBL2 |
SFTPA2 |
GDC |
G |
CD69 |
CLEC4C |
FCER2 |
MRC1 |
SFTPD |
PPC |
CD69 |
CLEC4C |
FCER2 |
MRC1 |
SFTPD |
PPC |
H |
CD72 |
CLEC4D |
KLRB1 |
MRC2 |
THBD |
NTC |
CD72 |
CLEC4D |
KLRB1 |
MRC2 |
THBD |
NTC |
* gene selection may be updated based on new research and development
货号 | GK111 |
产地 | 美国 |
缩写 | GQH-LRI |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |