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人类炎症信号和响应qPCR检测试剂盒

英文名:Human Inflammation Signaling and Response qPCR Array Kit

货号:GK108

价格:$250

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ScienCell's GeneQuery™ Human Inflammation Signaling and Response qPCR Array Kit (GQH-INF) surveys 88 key genes involved in the inflammatory response during wound healing.  Acute inflammation is one of the first immune responses to harmful stimuli and involves a complex network of signal transduction mediators.  When the immune system triggers an inflammatory response despite the lack of harmful stimuli, chronic inflammation can occur leading to diseases such as rheumatoid arthritis, hay fever, allergies, and sarcoidosis.  Brief examples of how included genes may be categorized are shown below:

  • Pattern recognition receptors: CRP, SFTPD, TIRAP, TLR2, TLR4
  • Cytokine production: INS, NFAM1, NOX5, S100B, TLR6
  • Cytokine metabolism: APOA2, CD4, CD86, TLR1, TLR3
  • Signal transduction elements: CAST, HRH1, PROCR, RIPK2, SERPINA1, SYK
  • Transcription factors: CEBPB, FOS, IRF4, IRF7, NR3C1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Inflammation Signaling and Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AIF1

C5AR1

CD86

FOS

IL1RAPL1

KITLG

NFATC3

PROCR

SELE

TACR1

TLR5

β-actin

B

APOA2

CAST

CEBPB

FPR1

IL1RN

LTB4R

NFATC4

PROK2

SERPINA1

THPO

TLR6

GAPDH

C

APOL2

CD14

CRP

GREM1

IL36RN

LY75

NFKB1

PTAFR

SEPINF1

TIRAP

TLR7

LDHA

D

BCL6

CD180

ERBB2

HDAC5

IL6ST

LY86

NOS2

PTGS2

SFTPD

TLR1

TLR8

NONO

E

BLNK

CD27

F11R

HDAC9

INS

LY96

NOX5

PTX3

SIGLEC1

TLR10

TLR9

PPIH

F

C3

CD28

F2

HRH1

IRF4

MYD88

NR3C1

RIPK2

STAB1

TLR2

TNFAIP6

GDC

G

C3AR1

CD4

F8

IL18RAP

IRF7

NCR3

PARP1

S100B

STAT3

TLR3

VEGFA

PPC

H

C5

CD74

FN1

IL1RAP

ITGB2

NFAM1

PLA2G7

SDCBP

SYK

TLR4

VPS45

NTC

 

 * gene selection may be updated based on new research and development

货号GK108
产地美国
缩写GQH-INF
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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