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人类细胞骨架重塑qPCR检测试剂盒

英文名:Human Cytoskeletal Remodeling qPCR Array Kit

货号:GK104

价格:$250

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ScienCell's GeneQuery™ Human Cytoskeletal Remodeling qPCR Array Kit (GQH-CYT) profiles 88 key genes involved in cytoskeletal remodeling with a particular focus on Rho GTPase actin cytoskeleton remodeling.  The cytoskeleton is responsible for maintaining cell shape, scaffolding, and regulating intracellular transport.  It is also involved in numerous signaling pathways, cellular division, vesicular trafficking, and motility.  Microfilaments, which are primarily actin polymers, are part of the cytoskeleton and regulated by the Rho family of GTPases.  Brief examples of how included genes may be categorized are shown below:

  • Cytoskeletal components: ACTA1, ACTB, ACTG1, ACTN1, MYH10, MYL1
  • Integrins: ITGA1, ITGA3, ITGAV, ITGB1, ITGB3
  • Extracellular matrix components: COL1A1, COL2A1, COL4A1, LAMA1, LAMB1
  • Rho signaling molecules: CAV1, CDC42, RAC1, RHOA, ROCK1, ROCK2
  • Transcription factors: CDX1, CDX2, SP1, TCF7, TCF7L1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Cytoskeletal Remodeling qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

ACTN3

CFL1

COL4A1

ITGA3

LAMB1

MYH2

MYL12B

MYLK

PTK2

SRC

β-actin

B

ACTA2

ACTN4

CFL2

COL4A2

ITGA5

LAMC1

MYH3

MYL2

MYLK2

PXN

TCF7

GAPDH

C

ACTB

ACTR3B

CLDN4

COL4A5

ITGA7

LEF1

MYH6

MYL3

MYLK3

RAC1

TCF7L1

LDHA

D

ACTC1

ARHGDIA

CLDN5

FLNA

ITGA8

LIMK1

MYH7

MYL4

MYLPF

RHOA

TCF7L2

NONO

E

ACTG1

CAV1

CLDN7

FN1

ITGAV

MAPK1

MYH7B

MYL5

NECTIN1

ROCK1

TJP1

PPIH

F

ACTG2

CDC42

COL1A1

GRB2

ITGB1

MAPK3

MYH8

MYL6

NKX2-1

ROCK2

TLN1

GDC

G

ACTN1

CDX1

COL1A2

ITGA1

ITGB3

MYH10

MYH9

MYL6B

PAK1

SNAI1

TLN2

PPC

H

ACTN2

CDX2

COL2A1

ITGA2

LAMA1

MYH11

MYL1

MYL9

PIP5K1C

SP1

VCL

NTC

 

 * gene selection may be updated based on new research and development

货号GK104
产地美国
缩写GQH-CYT
规格One 96-well plate, one 20ul reaction per well
用途科研
储存 4°C
运输胶冰
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