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人前脂肪细胞生物学qPCR检测试剂盒

英文名:Human Preadipocyte Cell Biology qPCR Array Kit

货号:GK103

价格:$250

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Preadipocytes are fibroblast-like precursor cells that can proliferate and differentiate into mature adipocytes. Preadipocytes play an important role in metabolic homeostasis and nutrient signaling. ScienCell's GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Kit (GQH-PAD) is designed to facilitate gene expression profiling of 88 key genes involved in human preadipocyte cell biology, such as cell proliferation, differentiation, ECM formation and lipid metabolism. Genes implicated in obesity and liposarcoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Preadipocyte cell markers: ADIPOQ, LEP, HOXC8/9, TBX1, TMEM26, UCP1, CIDEA, PRDM16, SLC7A10, P2RX5
  • Proliferation: CCND1, PROKR1, LYRM1, FTO, SLC2A4, PDGFA, IL4, IGF1, FGF5, MAPK1/3, TNFSF10
  • Differentiation and adipogenesis: PPARG, CEBPA/B, KLF2/5/15, SOCS1/3, INSR, DLK1, ADD1, SREBF1
  • ECM and cytoskeleton: MMP1/2/3, FAP, DPP4, TGFB1, COL1A1/2, SMAD2/4
  • Lipid metabolism: FABP5/7, LBP, LPL, PLIN2, ACSL1, LIPC
  • Obesity: MC4R, UCP3, ADRB2/3, POMC, FFAR4, CARTPT
  • Liposarcoma: FUS, MDM2, HMGA2, DDIT3, CDK4, EWSR1, DES, VIM

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

 

GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Plate Layout *

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACSL1

CDK4

DES

FAP

HMGA2

KLF15

LTBP1

MMP3

PRDM16

SLC7A10

TMEM26

ACTB

B

ADD1

CEBPA

DGAT2

FASLG

HOXC8

KLF2

LYRM1

P2RX5

PROKR1

SMAD2

TNF

GAPDH

C

ADIPOQ

CEBPB

DLK1

FFAR4

HOXC9

KLF5

MAPK1

PDGFA

PTGR1

SMAD4

TNFSF10

LDHA

D

ADRB2

CIDEA

DPP4

FGF5

IGF1

LBP

MAPK3

PDGFRA

RUNX2

SOCS1

UCP1

NONO

E

ADRB3

COL1A1

EWSR1

FTO

IL4

LEP

MC4R

PLIN2

SDC3

SOCS3

UCP3

PPIH

F

AGRP

COL1A2

FABP4

FUS

INPPL1

LIPC

MDM2

PNPLA2

SIM1

SREBF1

VIM

GDC

G

CARTPT

COL6A3

FABP5

GATA3

INSR

LIPE

MMP1

POMC

SLC2A4

TBX1

WISP2

PPC

H

CCND1

DDIT3

FABP7

GLI3

KITLG

LPL

MMP2

PPARG

SLC36A2

TGFB1

WNT5A

NTC

 

* gene selection may be updated based on new research and development

货号GK103
产地美国
缩写GQH-PAD
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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