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人类Synoviocyte细胞生物学qPCR检测试剂盒

英文名:Human Synoviocyte Cell Biology qPCR Array Kit

货号:GK101

价格:$250

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ScienCell's GeneQuery™ Human Synoviocyte Cell Biology qPCR Array Kit (GQH-SYN) is designed to facilitate gene expression profiling of 88 key genes involved in human synoviocyte cell biology, including synoviocyte markers, proliferation and invasion, extracellular matrix (ECM) and synovial fluid synthesis. Genes implicated in rheumatoid arthritis and synovial sarcoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Synoviocyte markers: CD55, CD14, MS4A1, ICAM1, ITGB1, CD40, CR2, VCAM1, DRC1, CDH11, THY1, VIM
  • Proliferation and invasion: NGF, TNFSF13, MIF, ESM1, EREG, IL1B, TNF, SAAL1, CDK6, POSTN, TWIST1, TRIM28, SMAD7, CCL2/5, SYVN1
  • Synovial fluid synthesis: PRG4, HP, IGF1, UGDH, PTGS1/2, PTGES, HAS1, ALB, TF, IFNG, IL6
  • Cell adhesion and ECM synthesis: CDH11, ITGAL, VCAM1, ITGA4, ADM, VTN, FN1, COL1A1, RAMP2/3, MMP1/8, AKT1, COMP
  • Genes implicated in
    • rheumatoid arthritis: LBH, PTPN22, SLC22A4, CIITA, PADI4, HLA-DRB1, CD244, IL10, SH2D1A, PREP
    • synovial sarcoma: SSX1/2/3/4, SS18, SYT1, FLI1, MUC1, HMGA2, IGF1R, MDM2, CTAG1B

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Synoviocyte Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADM

CD40

CSF2

FLI1

IGF1

ITGAL

MMP8

PTEN

SH2D1A

SYT1

TP53

ACTB

B

AKT1

CD55

CSNK1D

FN1

IGF1R

ITGB1

MS4A1

PTGES

SLC22A4

SYVN1

TRIM28

GAPDH

C

ALB

CDH11

CTAG1B

HAS1

IL10

KRT7

MUC1

PTGS1

SMAD7

TF

TWIST1

LDHA

D

CALCRL

CDK6

CXCL10

HLA-DRB1

IL15

LBH

NGF

PTGS2

SS18

TGFB1I1

UGDH

NONO

E

CCL2

CIITA

CXCL8

HMGA2

IL1B

MDM2

PADI4

PTPN22

SSX1

THY1

VCAM1

PPIH

F

CCL5

COL1A1

DRC1

HP

IL1RN

MIF

POSTN

RAMP2

SSX2

TIMP1

VIM

GDC

G

CD14

COMP

EREG

ICAM1

IL6

MMP1

PREP

RAMP3

SSX3

TNF

VTN

PPC

H

CD244

CR2

ESM1

IFNG

ITGA4

MMP3

PRG4

SAAL1

SSX4

TNFSF13

YAP1

NTC

 

* gene selection may be updated based on new research and development

货号GK101
产地美国
缩写GQH-SYN
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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