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人角质形成细胞生物学qPCR检测试剂盒

英文名:Human Keratinocyte Cell Biology qPCR Array Kit

货号:GK100

价格:$250

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ScienCell's GeneQuery™ Human Keratinocyte Cell Biology qPCR Array Kit (GQH-KCB) is designed to facilitate gene expression profiling of 88 key genes involved in human keratinocyte cell biology, including keratinocyte proliferation and differentiation, extracellular matrix (ECM) synthesis, cell adhesion and epithelialization. Genes implicated in skin carcinogenesis and psoriasis are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Keratinocyte cell markers: KRT10, KRT14, IVL, BNC1, TP63, KRTDAP
  • Proliferation and differentiation: KRT1/8/10/13/18, IVL, FLG, FLG2, LOR, TGM1, CDK1, CTNNB1, IL1B, CYR61
  • Migration and epithelialization: EGFR, HGF, MET, EGR3, AKAP12, PTGS2, LAMA3, LAMB3, LAMC2, FGFR1/2, IGF1, EGF
  • ECM synthesis: COL1A1/2, COL4A1/2, COL7A1, FN1, VTN, Laminins
  • Cell adhesion: CDH1/2/3/15, DSC1/2, DSG1/3, ITGA2, ITGB1, SELE/P, ICAM1/3, VCAM1, ITGA3/6, ITGB4
  • Skin carcinogenesis: PTCH1/2, SMO, TP53, GLI1/2, SHH, MC1R, CTNNB1, KRT15/17/20
  • Psoriasis: HLA-C, CDSN, IL36RN, TRAF3IP2, PSORS1C1/2, FLG, FABP5

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Keratinocyte Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKAP12

CDSN

DSC1

FGFR1

HLA-C

ITGA2

KRT1

KRT20

LAMC1

PSORS1C2

TGM1

ACTB

B

BNC1

COL1A1

DSC2

FGFR2

ICAM1

ITGA3

KRT10

KRT8

LAMC2

PTCH1

TP53

GAPDH

C

CCL2

COL1A2

DSG1

FLG

ICAM3

ITGA6

KRT13

KRTDAP

LOR

PTCH2

TP63

LDHA

D

CDH1

COL4A1

DSG3

FLG2

IGF1

ITGB1

KRT14

LAMA1

MC1R

PTGS2

TRAF3IP2

NONO

E

CDH15

COL4A2

EGF

FN1

IGF1R

ITGB1BP1

KRT15

LAMA3

MET

SELE

VCAM1

PPIH

F

CDH2

COL7A1

EGFR

GLI1

IL1B

ITGB4

KRT16

LAMB1

MYC

SELP

VTN

GDC

G

CDH3

CTNNB1

EGR3

GLI2

IL20

IVL

KRT17

LAMB2

NOTCH1

SHH

WNT1

PPC

H

CDK1

CYR61

FABP5

HGF

IL36RN

JAG1

KRT18

LAMB3

PSORS1C1

SMO

WNT3A

NTC

 

* gene selection may be updated based on new research and development

货号GK100
产地美国
缩写GQH-KCB
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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