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人类骨骼肌细胞生物学qPCR检测试剂盒

英文名:Human Skeletal Muscle Cell Biology qPCR Array Kit

货号:GK099

价格:$250

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ScienCell's GeneQuery™ Human Skeletal Muscle Cell Biology qPCR Array Kit (GQH-SKM) is designed to facilitate gene expression profiling of 88 key genes involved in human skeletal muscle cell biology, including skeletal muscle contractility, development, proliferation, and differentiation. Genes implicated in selected skeletal muscular disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Skeletal muscle cell markers: SGCA, CKM, EIF5A, ENO2, FABP3, MSTN, ITGA7, ITGB1, MCAM, MYOD1, MYOG, NCAM1, TNNI1
  • Skeletal muscle contractility: ACTA1, TTN, TCAP, MYH3, TNNT3, SLC2A1/4, TPM2, CPT1B, CPT2, PDHA1/2, PDHB, CACNA1F, DMD
  • Skeletal muscle cell development and proliferation: MYH3/8, ACTA1, PAX3/7, SIX1/4, MYF5/6, RPS6KB1, PITX2, BCL2, ZNF513, MYOD1, MYOG, EYA1/2
  • Skeletal muscle cell differentiation: MYOD1, MYF5/6, MYOG, TCF12, TRIM28, MEF2A/C,  HDAC1, EZH2, SRF, JUNB, FOXO3
  • Genes implicated in
    • muscular atrophy: AR, MTM1, ACTA1, TPM2, MYH2/7, CRYAB, GNE, DES, RYR1
    • muscular dystrophy: DMD, BEST1, UTRN, LTBP4, OTC, GK, LMNA, EMD, FHL1, SYNE2, TMEM43

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Skeletal Muscle Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

CAPN3

EHMT2

FABP3

HK2

MCAM

MYH2

PAX3

RPS6KA5

SLC2A4

TPM2

β-actin

B

AKT1

CKM

EIF5A

FHL1

ITGA7

MEF2A 

MYH3

PAX7

RPS6KB1

SRF

TRIM28

GAPDH

C

AR

CPT1B

EMD

FOXO3

ITGB1

MEF2C

MYH7

PDHA1

RYR1

SYNE2

TTN

LDHA

D

ATP2A1

CPT2

ENO2

FZD1

JSRP1

MSC

MYH8

PDHA2

SETDB1

TCAP

UNC93A

NONO

E

BCL2

CRYAB

EP300

FZD7

JUNB

MSTN

MYOD1

PDHB

SGCA

TCF12

UTRN

PPIH

F

BEST1

DES

EYA1

GK

KDM1A

MTM1

MYOG

PDK4

SIX1

TMEM43

WNT1

GDC

G

BMP4

DMD

EYA2

GNE

LMNA

MYF5

NCAM1

PITX2

SIX4

TNNI1

WNT3A

PPC

H

CACNA1F

DMPK

EZH2

HDAC1

LTBP4

MYF6

OTC

PKM

SLC2A1

TNNT3

ZNF513

NTC

 

* gene selection may be updated based on new research and development

 

货号GK099
产地美国
缩写GQH-SKM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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