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人类心脏肌细胞生物学qPCR检测试剂盒

英文名:Human Cardiac Myocyte Biology qPCR Array Kit

货号:GK098

价格:$250

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ScienCell's GeneQuery™ Human Cardiac Myocyte Biology qPCR Array Kit (GQH-CMB) is designed to facilitate gene expression profiling of 88 key genes involved in human cardiac myocyte biology, such as cardiac muscle contraction, cardiac myocyte proliferation and differentiation, and cardiac z-disk signaling network. Genes implicated in selected cardiac myocyte related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Cardiac myocyte markers: ACTN1, TNNT2, NKX2-5, GJA1, NPPA, MYH6, MYH7B, TNNI3, CAV3, GATA4, MYL2, MEF2C
  • Cardiac muscle contraction: CAMK2s, ATP2As, MYH7, MYL2, CACNA1C, HRC, ITPR3, KCNJ5, TRDN, TPMs
  • Cardiac z-disk signaling network: AGTR1, ACTNs, CSRP3, MYOZ2, ITGB1BP2, DES, ITGA1, ITGB1, ILK, CACNA1s, CIB1, MYOZ2/3
  • Cardiac myocyte development and proliferation: GATA4, HAND2, MEF2C, TBX5, FOXP1, NKX2-5, FGFs, PIK3CB, SAV1, STK3/4, YAP1, WWTR1
  • Cardiac myocyte differentiation: MYOCD, TWIST1, VANGL2, BCKDHA, CPT1B, GPAM, POU5F1, MEF2C, NPPA/B, MYOG
  • Genes implicated in
    • hypertrophic cardiomyopathy: MYH6/7, MYBPC3, TNNI3, PMPCA, TNNT2, TPM1, ACTC1, MYL2/3
    • myocardial infarction: OLR1, LRP8, LTA, LGALS2, ESR1, FABP6

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Cardiac Myocyte Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTN1

ATP2A3

CAMK2D

FABP6

GLRX3

ITPR3

MYBPC3

MYOZ2

PMPCA

SLC9A1

TPM2

β-actin

B

ACTN2

BCKDHA

CAMK2G

FGF10

GPAM

KAT2B

MYH6

MYOZ3

POU5F1

STK3

TPM3

GAPDH

C

ACTC1

CACNA1C

CAV3

FGF16

HAND2

KCNJ5

MYH7

NKX2-5

PRKCB

STK4

TPM4

LDHA

D

ACTN3

CACNA1D

CIB1

FGF20

HRC

LGALS2

MYH7B

NPPA

QDPR

SYNPO2

TRDN

NONO

E

ACTN4

CACNA1F

CPT1B

FGF3

ILK

LMCD1

MYL2

NPPB

RYR2

TBX5

TWIST1

PPIH

F

AGTR1

CACNA1S

CSRP3

FOXP1

ITGA1

LRP8

MYL3

OLR1

SAV1

TNNI3

VANGL2

GDC

G

ATP2A1

CAMK2A

DES

GATA4

ITGB1

LTA

MYOCD

PAK1

SCN5A

TNNT2

WWTR1

PPC

H

ATP2A2

CAMK2B

ESR1

GJA1

ITGB1BP2

MEF2C

MYOG

PIK3CB

SLC8A1

TPM1

YAP1

NTC

 

* gene selection may be updated based on new research and development

货号GK098
产地美国
缩写GQH-CMB
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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