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人类平滑肌细胞生物学qPCR检测试剂盒

英文名:Human Smooth Muscle Cell Biology qPCR Array Kit

货号:GK097

价格:$250

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ScienCell's GeneQuery™ Human Smooth Muscle Cell Biology qPCR Array Kit (GQH-SMC) is designed to facilitate gene expression profiling of 88 key genes involved in human smooth muscle cell (SMC) biology, such as vascular SMC contraction, SMC phenotypic switching, and the extracellular matrix (ECM) synthesis. Genes implicated in selected SMC related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • SMC markers: ACTA2, CDH5, CALD1, HEXIM1, HRH2, MLNR, TAGLN, DES, CSPG4, SMTN, MKL1/2
  • Vascular SMC contraction: ANO1, ADRA1s, NPR1/2, GNAs, MYLKs, ADORA2s, EDNRA, MYH11, MYLs
  • SMC phenotypic switching: MYH11, MEF2B, SRF, ELK1, MYOCD, GATA6, MKL1/2, RHOA, TGFB1, NOTCH1/3, HEY2
  • SMC heterogeneity: ACTA2, TAGLN, CNN2, MYH11, OGN, AQP1, PLN, MGP, SPP1, KDR, SMTN, VCL, DES
  • ECM synthesis: LAMA5, collagens, ELN, OGN, MGP, SPP1, FGA/B/G
  • Genes implicated in
    • systemic hypertension: PTGIS, AGT, ADD1, AGTR1, GNB3, NOS3
    • achalasia: NOS1, AAAS, GMPPA, GUCY1A3, HLA-DQA1/B1
    • bronchospasm/asthma: HNMT, IL13, CCL11, ADRB2, ALOX5, SCGB3A2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Smooth Muscle Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AAAS

ADRB2

AVPR1B

COL3A1

ELN

GNA13

HRH2

MLNR

MYOCD

NPR2

SCGB3A2

β-actin

B

ACTA2

AEBP1

CALCRL

COL4A1

FGA

GNB3

IL13

MYH11

NKX3-1

OGN

SMAD3

GAPDH

C

ADD1

AGT

CALD1

CSPG4

FGB

GUCY1A3

KDR

MYL6

NOS1

PLN

SMTN

LDHA

D

ADORA2A

AGTR1

CCL11

CYP4A11

FGG

HEXIM1

LAMA5

MYL6B

NOS3

PTGIR

SPP1

NONO

E

ADORA2B

ALOX5

CDH5

CYP4A22

GATA6

HEY2

MEF2B

MYL9

NOTCH1

PTGIS

SRF

PPIH

F

ADRA1A

ANO1

CNN2

DES

GMPPA

HLA-DQA1

MGP

MYLK

NOTCH3

RBP1

TAGLN

GDC

G

ADRA1B

AQP1

COL1A1

EDNRA

GNA11

HLA-DQB1

MKL1

MYLK2

NOX4

RBPJ

TGFB1

PPC

H

ADRA1D

AVPR1A

COL1A2

ELK1

GNA12

HNMT

MKL2

MYLK3

NPR1

RHOA

VCL

NTC

 

* gene selection may be updated based on new research and development

货号GK097
产地美国
缩写GQH-SMC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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