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人类雪旺细胞生物学qPCR检测试剂盒

英文名:Human Schwann Cell Biology qPCR Array Kit

货号:GK096

价格:$250

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ScienCell's GeneQuery™ Human Schwann Cell Biology qPCR Array Kit (GQH-SCH) is designed to facilitate gene expression profiling of 88 key genes involved in human Schwann cell biology, such as peripheral nerve formation and regeneration, Schwann cell development and differentiation, myelination and extracellular matrix (ECM) synthesis. Genes implicated in selected Schwann cell related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Schwann cell markers: SOX10, NGFR, SOX2, GAP43, NCAM1, EGR2, POU3F1, MBP, MPZ, S100B, PMP22, GFAP
  • Schwann cell myelination: MOG, CNP, SOX10, EGR2, NFATC4, NRG1, CD9, HEXB, IFNG, NTF3, PLP1
  • Maturation and differentiation: WASL, NCAM1, VGF, IL6, BDNF, STAT1, PTN, CD40, LIF, MEF2C
  • Schwann cell plasticity and dedifferentiation: ERBB2, RAF1, MAPK1, MAPK3, MAPK11, MAPK14, JUN, ARTN, BDNF, GDNF, SOX2, GJA1
  • Nerve regeneration support: NTN1, UNC5B, SHH, GAP43, CDH1, TYRP1, FABP7, SOSTDC1, KCNJ10, LRRTM4, OLIG1
  • ECM synthesis in the peripheral nerve trunk: laminins, collagens, FN1, ICAM1, PLAT, PLAUR, NRG1, ITGB1, ERBB2, ERBB3
  • Genes implicated in
    • hereditary motor and sensory neuropathies: WNK1, KIF1A, FAM134B, SCN9A, APOB, IKBKAP, IFNB1, HLAs, AQP4, PLP1, IFNG
    • schwannoma: NF1, NF2, SMARCB1, KIT, PRKAR1A, GFAP, EZR, SOX10, NGFR, MBP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Schwann Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

APOB

CNP

ERBB3

GJA1

IFNG

LAMA1

MAPK11

NCAM1

OLIG1

RAF1

SOX2

β-actin

B

AQP4

COL1A1

EZR

GZMB

IGFBP2

LAMA2

MAPK14

NF1

PLAT

S100B

STAT1

GAPDH

C

ARTN

COL1A2

FABP7

HEXB

IKBKAP

LAMB1

MAPK3

NF2

PLAUR

SCN9A

TGFB1

LDHA

D

BDNF

COL3A1

FAM134B

HLA-DQA1

IL6

LAMB2

MBP

NFATC4

PLP1

SHH

TYRP1

NONO

E

CD40

COL4A1

FN1

HLA-DQB1

ITGB1

LIF

MEF2C

NGFR

PMP22

SMARCB1

UNC5B

PPIH

F

CD9

COL4A2

GAP43

HLA-DRB1

JUN

LRRTM4

MOG

NRG1

POU3F1

SOD1

VGF

GDC

G

CDH1

EGR2

GDNF

ICAM1

KCNJ10

MAL

MPZ

NTF3

PRKAR1A

SOSTDC1

WASL

PPC

H

CHST8

ERBB2

GFAP

IFNB1

KIT

MAPK1

MYRF

NTN1

PTN

SOX10

WNK1

NTC

* gene selection may be updated based on new research and development

货号GK096
产地美国
缩写GQH-SCH
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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