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人间充质至上皮细胞转化qPCR检测试剂盒

英文名:Human Mesenchymal to Epithelial Transition qPCR Array Kit

货号:GK095

价格:$250

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ScienCell's GeneQuery™ Human Mesenchymal To Epithelial Transition qPCR Array Kit (GQH-MET) is designed to facilitate gene expression profiling of 88 key genes involved in reversible mesenchymal to epithelial transition (MET). MET and the reverse process, epithelial to mesenchymal transition (EMT), are involved in embryogenesis, cancer metastasis, and stem cell reprogramming. Brief examples of how genes may be grouped are shown below:     

  • EMT markers: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD
  • EMT-MET transition: PAX2, WT1, BMP7, TWIST1, PRRX1
  • Proliferation and morphogenesis control: AKT1, BMP1, BMP7, EGFR, FGFBP1, IGFBP4, JAG1, PDGFRB, SNAI1, SOX10, VCAN, WNT5A, WNT11, ZEB1
  • Differentiation control: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, NODAL, SMAD2, SOX10, WNT5B
  • Transcription factors regulating MET: CTNNB1, ESR1, FOXC2, NOTCH1, SMAD2, SNAI1/2/3, STAT3, TCF3, TCF4, TWIST1, ZEB2
  • Signaling pathways involved in MET
    • Estrogen receptor: CAV2, ESR1, KRT19, TGFB3
    • GPCR: AKT1, FZD7, GNG11, RAC1, RGS2
    • PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Mesenchymal To Epithelial Transition qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CAV2

COL3A1

F11R

IGFBP4

MUC1

PIK3CG

RGS2

SNAI3

TGFB2

VCAN

β-actin

B

AKT1

CDH1

COL5A2

FGFBP1

ILK

NODAL

PIK3R1

RPS27A

SOX10

TGFB3

VIM

GAPDH

C

AKT2

CDH11

CTNNB1

FN1

JAG1

NOTCH1

PIK3R3

S100A4

SPINT2

TGFBR1

WNT11

LDHA

D

ARHGEF18

CDH2

DSP

FOXC2

KRT14

PAX2

PIK3R5

SDC1

STAT3

TGFBR2

WNT5A

NONO

E

AXL

CDKN2A

EGFR

FZD7

KRT19

PDGFD

PRKCZ

SMAD2

STK11

TIMP1

WNT5B

PPIH

F

BMP1

CDKN2B

ERBB3

GNG11

LEF1

PDGFRB

PRRX1

SMURF1

TCF3

TRIM28

WT1

GDC

G

BMP2

CLDN4

ESR1

GSC

MMP2

PIK3CA

RAB25

SNAI1

TCF4

TWIST1

ZEB1

PPC

H

BMP7

CLDN7

ETS1

HNRNPAB

MST1R

PIK3CD

RAC1

SNAI2

TGFB1

UBA52

ZEB2

NTC

* gene selection may be updated based on new research and development

货号GK095
产地美国
缩写GQH-MET
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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